Evidence map›Paper›PMID 33795743›Full record

ArticleScientific reports2021

Skeletal muscle fibers play a functional role in host defense during sepsis in mice.

Orlando Laitano, Gerard P Robinson, Kevin O Murray, Christian K Garcia, Alex J Mattingly, Deborah Morse, Michelle A King, John D Iwaniec, Jamal M Alzahrani, Thomas L Clanton

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Orlando Laitano *Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Gerard P Robinson *Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Kevin O MurrayDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Christian K GarciaDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Alex J MattinglyDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Deborah MorseDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Michelle A KingDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
John D IwaniecDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Jamal M AlzahraniDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA.
Thomas L ClantonDepartment of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, 1864 Stadium Rd, Gainesville, FL, 32611, USA. tclanton@hhp.ufl.edu.
University of Florida · US

Funding

Breathing Research and Therapeutics (BREATHE)T32HL134621 · NHLBI · UNIVERSITY OF FLORIDA · PI Gordon S. Mitchell · 2017 to 2026
$3.4M
Functional Role of Skeletal Muscle in the Innate Immune to SepsisR01GM118895 · NIGMS · UNIVERSITY OF FLORIDA · PI CLANTON, THOMAS LINDSAY · 2016 to 2019
$1.3M
NHLBI NIH HHS T32 HL134621NIGMS NIH HHS R01 GM118895
6 · The paper itself

Abstract

Skeletal muscles secrete a wide variety of immunologically active cytokines, but the functional significance of this response to in vivo innate immunity is not understood. We addressed this by knocking out the toll receptor adapter protein, Myd88, only in skeletal muscle fibers (skmMyd88KO), and followed male and female mice at 6 and 12 h after peritoneal injection of cecal slurry (CS), a model of polymicrobial sepsis. Because of a previously identified increase in mortality to CS injection, males received ~ 30% lower dose. At 12 h, skmMyd88KO caused significant reductions in a wide variety of pro- and anti-inflammatory plasma cytokines, e.g. TNFα, IL-1β and IL-10, compared to strain-matched controls in both males and females. Similar reductions were observed at 6 h in females. SkmMyd88KO led to ~ 40-50% elevations in peritoneal neutrophils at 6 and 12 h post CS in females. At 12 h post CS, skmMyd88KO increased peritoneal monocytes/macrophages and decreased %eosinophils and %basophils in females. SkmMyd88KO also led to significantly higher rates of mortality in female mice but not in males. In conclusion, the results suggest that skeletal muscle Myd88-dependent signal transduction can play functionally important role in normal whole body, innate immune inflammatory responses to peritoneal sepsis.

Indexed as

Signal TransductionAnimalsCytokinesDisease Models, AnimalFemaleImmune SystemImmunity, InnateInflammationLeukocytesMacrophages, PeritonealMaleMiceMice, Inbred C57BLMice, KnockoutMuscle, SkeletalMyeloid Differentiation Factor 88CytokinesMyd88 protein, mouseMyeloid Differentiation Factor 88

Identifiers

PMID33795743
PMCPMC8016959
OpenAlexW3148160075

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.