Evidence map›Paper›PMID 33795704›Full record

ArticleScientific reports2021

Adsorption of bacteriophages on polypropylene labware affects the reproducibility of phage research.

Łukasz Richter, Karolina Księżarczyk, Karolina Paszkowska, Marta Janczuk-Richter, Joanna Niedziółka-Jönsson, Jacek Gapiński, Marcin Łoś, Robert Hołyst, Jan Paczesny

Abstract read
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Guideline
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  7. Refining Viral Production Estimation.Environmental microbiology reports · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Łukasz RichterInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Karolina KsiężarczykInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Karolina PaszkowskaInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Marta Janczuk-RichterInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Joanna Niedziółka-JönssonInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland.
Jacek GapińskiDepartment of Molecular Biophysics, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 2, 61-614, Poznań, Poland.
Marcin ŁośDepartment of Molecular Biology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdansk, Poland.
Robert HołystInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland. rholyst@ichf.edu.pl.
Jan PaczesnyInstitute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224, Warsaw, Poland. jpaczesny@ichf.edu.pl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hydrophobicity is one of the most critical factors governing the adsorption of molecules and objects, such as virions, on surfaces. Even moderate change of wetting angle of plastic surfaces causes a drastic decrease ranging from 2 to 5 logs of the viruses (e.g., T4 phage) in the suspension due to adsorption on polymer vials' walls. The effect varies immensely in seemingly identical containers but purchased from different vendors. Comparison of glass, polyethylene, polypropylene, and polystyrene containers revealed a threshold in the wetting angle of around 95°: virions adsorb on the surface of more hydrophobic containers, while in more hydrophilic vials, phage suspensions are stable. The polypropylene surface of the Eppendorf-type and Falcon-type can accommodate from around 10

Indexed as

AdsorptionBacteriophagesBacteriophage T4GlassHydrophobic and Hydrophilic InteractionsMicroscopy, Atomic ForceMicroscopy, Electron, ScanningPhotoelectron SpectroscopyPlasticsPolyethylenePolymersPolypropylenesPolysorbatesPolystyrenesReproducibility of ResultsSurface-Active AgentsPlasticsPolyethylenePolymersPolypropylenesPolysorbatesPolystyrenesSurface-Active Agents

Identifiers

PMID33795704
PMCPMC8016829

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.