ArticlePlant physiology2021
Ubiquitylome analysis reveals a central role for the ubiquitin-proteasome system in plant innate immunity.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Genomes of the ex-type strains of Elsinoë mangiferae and E. perseae, the causal agents of scab on mango and avocado.G3 (Bethesda, Md.) · 2026Article
- Ubiquitination-driven proteostasis remodeling underlies oxidative stress and actin degradation in citrus huanglongbing.Plant physiology · 2026Article
- Salicylic acid modulates its catabolic enzymes via proteasomal degradation linked to SCF-associated proximity networks.Nature communications · 2026Article
- A phosphorylation-dependent ubiquitination switch orchestrates nuclear immune reprogramming upon chitin perception.Nature communications · 2026Article
- Ubiquitin-Mediated Proteolysis as a Regulator of the Plant Defense-Growth Balance.Plants (Basel, Switzerland) · 2026Review
- Advances in the molecular mechanism of grapevine resistance to fungal diseases.Molecular horticulture · 2025Review
- Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.The Plant cell · 2024Article
- Protein Dynamics in Plant Immunity: Insights into Plant-Pest Interactions.International journal of molecular sciences · 2024Review
- Contrasting Impacts of Ubiquitin Overexpression on Arabidopsis Growth and Development.Plants (Basel, Switzerland) · 2024Article
- A dynamic ubiquitination balance of cell proliferation and endoreduplication regulators determines plant organ size.Science advances · 2024Article
- Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response toHorticulture research · 2024Article
- Symbiont-host interactome mapping reveals effector-targeted modulation of hormone networks and activation of growth promotion.Nature communications · 2023Article
- Paradigms of receptor kinase signaling in plants.The Biochemical journal · 2023Review
- iTRAQ based proteomic analysis of rice lines having single or stacked blast resistance genes:Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Alterations of phenotype, physiology, and functional substances reveal the chilling-tolerant mechanism in two commonFrontiers in plant science · 2023Article
- Proteasome Dysfunction Leads to Suppression of the Hypoxic Response Pathway in Arabidopsis.International journal of molecular sciences · 2022Article
- Comparative Seeds Storage Transcriptome Analysis ofInternational journal of molecular sciences · 2022Article
- Harnessing the ubiquitin code to respond to environmental cues.Essays in biochemistry · 2022Article
- Thirty years of resistance: Zig-zag through the plant immune system.The Plant cell · 2022Review
- Ubiquitination of Receptorsomes, Frontline of Plant Immunity.International journal of molecular sciences · 2022Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Protein ubiquitylation profoundly expands proteome functionality and diversifies cellular signaling processes, with recent studies providing ample evidence for its importance to plant immunity. To gain a proteome-wide appreciation of ubiquitylome dynamics during immune recognition, we employed a two-step affinity enrichment protocol based on a 6His-tagged ubiquitin (Ub) variant coupled with high sensitivity mass spectrometry to identify Arabidopsis proteins rapidly ubiquitylated upon plant perception of the microbe-associated molecular pattern (MAMP) peptide flg22. The catalog from 2-week-old seedlings treated for 30 min with flg22 contained 690 conjugates, 64 Ub footprints, and all seven types of Ub linkages, and included previously uncharacterized conjugates of immune components. In vivo ubiquitylation assays confirmed modification of several candidates upon immune elicitation, and revealed distinct modification patterns and dynamics for key immune components, including poly- and monoubiquitylation, as well as induced or reduced levels of ubiquitylation. Gene ontology and network analyses of the collection also uncovered rapid modification of the Ub-proteasome system itself, suggesting a critical auto-regulatory loop necessary for an effective MAMP-triggered immune response and subsequent disease resistance. Included targets were UBIQUITIN-CONJUGATING ENZYME 13 (UBC13) and proteasome component REGULATORY PARTICLE NON-ATPASE SUBUNIT 8b (RPN8b), whose subsequent biochemical and genetic analyses implied negative roles in immune elicitation. Collectively, our proteomic analyses further strengthened the connection between ubiquitylation and flg22-based immune signaling, identified components and pathways regulating plant immunity, and increased the database of ubiquitylated substrates in plants.
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