ArticlePlant physiology2021
The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 74 citations in OpenAlex.
- Identification of poplar and spruce GDP-mannose pyrophosphorylases involved in mannan and ascorbic acid biosynthesis.Tree physiology · 2026Article
- Natural and CRISPR/Cas9 Editing Variations of Dual-uORFs Synergistically Increase the Nutritional Value of Silage Maize Feed.Plant biotechnology journal · 2026Article
- Metabolic engineering for tobacco quality improvement: Advances and perspectives.Metabolic engineering communications · 2026Review
- GhGLDH35A gene-mediated ROS homeostasis and stomatal movement via the ascorbic acid pathway confers alkaline stress tolerance.Journal of advanced research · 2026Article
- Simultaneous knockout of VITAMIN C DEFECTIVE 2 and 3 exacerbates ascorbate deficiency and light stress sensitivity.Journal of experimental botany · 2025Article
- Metabolic engineering inaBIOTECH · 2025Review
- Origin and evolution of signaling pathways responsible for ascorbic acid synthesis and catabolism during plant terrestrialization.Horticulture research · 2025Article
- Genome-wide meta-QTL approach identifies consensus loci associated with agriculturally important tomato fruit traits.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.Journal of experimental botany · 2025Article
- A bHLH transcription factor RrUNE12 regulates salt tolerance and promotes ascorbate synthesis.Plant cell reports · 2025Article
- Genome-wide association studies in a diverse strawberry collection unveil loci controlling agronomic and fruit quality traits.The plant genome · 2024Article
- Ascorbic acid metabolism and functions.Journal of experimental botany · 2024Article
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Antioxidant production promotes defense mechanism and different gene expression level in Zea mays under abiotic stress.Scientific reports · 2024Article
- Article
- Effect of light on ascorbic acid biosynthesis and bioinformatics analysis of related genes in Chinese chives.PloS one · 2024Article
- GmPLP1 negatively regulates soybean resistance to high light stress by modulating photosynthetic capacity and reactive oxygen species accumulation in a blue light-dependent manner.Plant biotechnology journal · 2023Article
- Genome-wide association study unveils ascorbate regulation by PAS/LOV PROTEIN during high light acclimation.Plant physiology · 2023Article
- GhIMP10D, an inositol monophosphates family gene, enhances ascorbic acid and antioxidant enzyme activities to confer alkaline tolerance in Gossypium hirsutum L.BMC plant biology · 2023Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
The enzymes involved in l-ascorbate biosynthesis in photosynthetic organisms (the Smirnoff-Wheeler [SW] pathway) are well established. Here, we analyzed their subcellular localizations and potential physical interactions and assessed their role in the control of ascorbate synthesis. Transient expression of C terminal-tagged fusions of SW genes in Nicotiana benthamiana and Arabidopsis thaliana mutants complemented with genomic constructs showed that while GDP-d-mannose epimerase is cytosolic, all the enzymes from GDP-d-mannose pyrophosphorylase (GMP) to l-galactose dehydrogenase (l-GalDH) show a dual cytosolic/nuclear localization. All transgenic lines expressing functional SW protein green fluorescent protein fusions driven by their endogenous promoters showed a high accumulation of the fusion proteins, with the exception of those lines expressing GDP-l-galactose phosphorylase (GGP) protein, which had very low abundance. Transient expression of individual or combinations of SW pathway enzymes in N. benthamiana only increased ascorbate concentration if GGP was included. Although we did not detect direct interaction between the different enzymes of the pathway using yeast-two hybrid analysis, consecutive SW enzymes, as well as the first and last enzymes (GMP and l-GalDH) associated in coimmunoprecipitation studies. This association was supported by gel filtration chromatography, showing the presence of SW proteins in high-molecular weight fractions. Finally, metabolic control analysis incorporating known kinetic characteristics showed that previously reported feedback repression at the GGP step, combined with its relatively low abundance, confers a high-flux control coefficient and rationalizes why manipulation of other enzymes has little effect on ascorbate concentration.
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