ArticlePlant physiology2021
Molecular basis for high ligand sensitivity and selectivity of strigolactone receptors in Striga.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- In vitro dynamic and quantitative monitoring of strigolactone-signaling complex formation by time-resolved FRET.The Plant journal : for cell and molecular biology · 2025Article
- Mechanism of cooperative strigolactone perception by the MAX2 ubiquitin ligase-receptor-substrate complex.Nature communications · 2025Article
- Mechanistic basis for enhanced strigolactone sensitivity in KAI2 triple mutant.Biophysical journal · 2025Article
- Development of Dispersive Liquid-Liquid Microextraction Method Based on Solidification of Floating Organic Droplets for Rapid Determination of Three Strigolactones in Rice (International journal of molecular sciences · 2025Article
- Cracking the enigma: understanding strigolactone signalling in the rhizosphere.Journal of experimental botany · 2024Review
- A Divergent Clade KAI2 Protein in the Root Parasitic Plant Orobanche minor Is a Highly Sensitive Strigolactone Receptor and Is Involved in the Perception of Sesquiterpene Lactones.Plant & cell physiology · 2023Article
- Role of Strigolactones in the Host Specificity of Broomrapes and Witchweeds.Plant & cell physiology · 2023Review
- Diversification of plant SUPPRESSOR OF MAX2 1 (SMAX1)-like genes and genome-wide identification and characterization of cotton SMXL gene family.BMC plant biology · 2023Article
- Lessons from a century of apical dominance research.Journal of experimental botany · 2023Article
- Structural analysis of a hormone-bound Striga strigolactone receptor.Nature plants · 2023Article
- Effect of histidine covalent modification on strigolactone receptor activation and selectivity.Biophysical journal · 2023Article
- A KARRIKIN INSENSITIVE2 paralog in lettuce mediates highly sensitive germination responses to karrikinolide.Plant physiology · 2022Article
- Crystal structure ofPlant direct · 2022Article
- A conformational switch in the SCF-D3/MAX2 ubiquitin ligase facilitates strigolactone signalling.Nature plants · 2022Article
- A novel strigolactone receptor antagonist provides insights into the structural inhibition, conditioning, and germination of the crop parasite Striga.The Journal of biological chemistry · 2022Article
- The strigolactone receptor D14 targets SMAX1 for degradation in response to GR24 treatment and osmotic stress.Plant communications · 2022Article
- Rapid analysis of strigolactone receptor activity in aPlant direct · 2022Article
- Three mutations repurpose a plant karrikin receptor to a strigolactone receptor.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Parasitic plants: physiology, development, signaling, and ecosystem interactions.Plant physiology · 2021Article
- The mechanism of host-induced germination in root parasitic plants.Plant physiology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Seeds of the root parasitic plant Striga hermonthica can sense very low concentrations of strigolactones (SLs) exuded from host roots. The S. hermonthica hyposensitive to light (ShHTL) proteins are putative SL receptors, among which ShHTL7 reportedly confers sensitivity to picomolar levels of SL when expressed in Arabidopsis thaliana. However, the molecular mechanism underlying ShHTL7 sensitivity is unknown. Here we determined the ShHTL7 crystal structure and quantified its interactions with various SLs and key interacting proteins. We established that ShHTL7 has an active-site pocket with broad-spectrum response to different SLs and moderate affinity. However, in contrast to other ShHTLs, we observed particularly high affinity of ShHTL7 for F-box protein AtMAX2. Furthermore, ShHTL7 interacted with AtMAX2 and with transcriptional regulator AtSMAX1 in response to nanomolar SL concentration. ShHTL7 mutagenesis analyses identified surface residues that contribute to its high-affinity binding to AtMAX2 and residues in the ligand binding pocket that confer broad-spectrum response to SLs with various structures. Crucially, yeast-three hybrid experiments showed that AtMAX2 confers responsiveness of the ShHTL7-AtSMAX1 interaction to picomolar levels of SL in line with the previously reported physiological sensitivity. These findings highlight the key role of SL-induced MAX2-ShHTL7-SMAX1 complex formation in determining the sensitivity to SL. Moreover, these data suggest a strategy to screen for compounds that could promote suicidal seed germination at physiologically relevant levels.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.