ArticlePlant physiology2021
Chloroplast-derived photo-oxidative stress causes changes in H2O2 and EGSH in other subcellular compartments.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 104 citations in OpenAlex.
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- PERSPECTIVE: organelle positioning as a principle of metabolic regulation and stress tolerance.The Plant journal : for cell and molecular biology · 2026Review
- Harnessing Antioxidants for Abiotic Stress Management: Mechanistic Insights and Prospects for Sustainable Agriculture.Antioxidants (Basel, Switzerland) · 2026Review
- Papaver S-determinants trigger mitochondrially derived ROS production and disrupt energy metabolism in incompatible pollen tubes.The Plant cell · 2026Article
- The secreted redox sensor roGFP2-Orp1 reveals oxidative dynamics in the plant apoplast.Plant biology (Stuttgart, Germany) · 2026Article
- Leaf it to science: Uncovering plant immune systems through technological advances.Plant physiology · 2026Review
- The redox rhythm gates immune-induced cell death distinctly from the genetic clock.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring the taste code of light-withered tea: the mechanism of nonvolatile metabolite changes in LED light-withered oolong tea (Food chemistry: X · 2025Article
- Redox buffering and HThe Plant journal : for cell and molecular biology · 2025Article
- Diatoms exhibit dynamic chloroplast calcium signals in response to high light and oxidative stress.Plant physiology · 2024Article
- Antioxidant Responses and Redox Regulation Within Plant-Beneficial Microbe Interaction.Antioxidants (Basel, Switzerland) · 2024Review
- Hydrogen peroxide participates in leaf senescence by inhibiting CHLI1 activity.Plant cell reports · 2024Article
- Arabidopsis transcription factor ANAC102 predominantly expresses a nuclear protein and acts as a negative regulator of methyl viologen-induced oxidative stress responses.Journal of experimental botany · 2024Article
- Hydrogen peroxide sulfenylates and inhibits the photorespiratory enzyme PGLP1 to modulate plant thermotolerance.Plant communications · 2024Article
- Antioxidant production promotes defense mechanism and different gene expression level in Zea mays under abiotic stress.Scientific reports · 2024Article
- Transcriptomic changes in barley leaves induced by alcohol ethoxylates indicate potential pathways of surfactant detoxification.Scientific reports · 2024Article
- Chloroplasts lacking class I glutaredoxins are functional but show a delayed recovery of protein cysteinyl redox state after oxidative challenge.Redox biology · 2024Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolic fluctuations in chloroplasts and mitochondria can trigger retrograde signals to modify nuclear gene expression. Mobile signals likely to be involved are reactive oxygen species (ROS), which can operate protein redox switches by oxidation of specific cysteine residues. Redox buffers, such as the highly reduced glutathione pool, serve as reservoirs of reducing power for several ROS-scavenging and ROS-induced damage repair pathways. Formation of glutathione disulfide and a shift of the glutathione redox potential (EGSH) toward less negative values is considered as hallmark of several stress conditions. Here we used the herbicide methyl viologen (MV) to generate ROS locally in chloroplasts of intact Arabidopsis (Arabidopsis thaliana) seedlings and recorded dynamic changes in EGSH and H2O2 levels with the genetically encoded biosensors Grx1-roGFP2 (for EGSH) and roGFP2-Orp1 (for H2O2) targeted to chloroplasts, the cytosol, or mitochondria. Treatment of seedlings with MV caused rapid oxidation in chloroplasts and, subsequently, in the cytosol and mitochondria. MV-induced oxidation was significantly boosted by illumination with actinic light, and largely abolished by inhibitors of photosynthetic electron transport. MV also induced autonomous oxidation in the mitochondrial matrix in an electron transport chain activity-dependent manner that was milder than the oxidation triggered in chloroplasts by the combination of MV and light. In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provides a basis for understanding how compartment-specific redox dynamics might operate in retrograde signaling and stress acclimation in plants.
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