ArticleThe Plant cell2021
An alternative splicing variant of PtRD26 delays leaf senescence by regulating multiple NAC transcription factors in Populus.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers, 1 of them a synthesis that pooled it.
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Who cites it
67 citing papers in PubMed, 1 synthesis or guideline pooled it, 159 citations in OpenAlex.
- Overview of molecular mechanisms of plant leaf development: a systematic review.Frontiers in plant science · 2023Pooled it
- Gene network analysis predicts the primary regulators of ABA-dependent transcriptional activation and repression in Populus roots.PLoS genetics · 2026Article
- Unraveling competitive protein interactions: A critical step toward improved production and therapeutics.Journal of advanced research · 2026Review
- The NAC transcription factor BrrNAC082 positively mediates leaf senescence in Brassica rapa.The Plant journal : for cell and molecular biology · 2026Article
- Alternative Splicing in Plant Development and Abiotic Stress Responses: A Multifunctional Regulatory Mechanism.International journal of molecular sciences · 2026Review
- Article
- Regulatory Mechanisms of Leaf Senescence in Herbaceous and Woody Perennials: A Comparative Review.Plants (Basel, Switzerland) · 2026Review
- mRNA decapping proteins regulate EIN2-dependent ethylene signaling in arabidopsis.Frontiers in plant science · 2026Article
- The NAC Transcription FactorsInternational journal of molecular sciences · 2025Article
- Genome-Wide Isoform Switching Reveals SR45-Mediated Splicing Control ofInternational journal of molecular sciences · 2025Article
- Genome-wide analysis of NAC gene family in Brassica campestris ssp. chinensis and characterization of BcNAC134.Plant cell reports · 2025Article
- A single-base mutation on the 5' UTR of BrATG5 confers the premature leaf senescence phenotype in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- SC35-mediated bZIP49 splicing regulates K⁺ channel AKT1 for salt stress adaptation in poplar.Nature communications · 2025Article
- Maize leaf yellowing gene ZmCAAX modulates growth and drought resistance by regulating abscisic acid contents through interaction with the ABA biosynthetic enzyme ZmNCED3.Plant biotechnology journal · 2025Article
- Alternative Splicing of Functional Genes in Plant Growth, Development, and Stress Responses.International journal of molecular sciences · 2025Review
- Genome-wide identification of NAC transcription factors in Acer paxii, and their expression dynamics during leaf aging.Genes & genomics · 2025Article
- Comprehensive analysis of the NAC transcription factor gene family in Sophora tonkinensis Gagnep.BMC plant biology · 2025Article
- Genome-Wide Identification, Characterization, and Expression Analysis of the NAC Transcription Factor Family in Sweet Cherry (Plants (Basel, Switzerland) · 2025Article
- NAC047/052/104 Synergistically Regulate the Dark-Induced Leaf Senescence in Non-Heading Chinese Cabbage.International journal of molecular sciences · 2025Article
- Comprehensive Genomic Analysis of Trihelix Transcription Factor Genes and Their Expression Underlying Abiotic Stress in Euphrates Poplar (Plants (Basel, Switzerland) · 2025Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During leaf senescence, the final stage of leaf development, nutrients are recycled from leaves to other organs, and therefore proper control of senescence is thus critical for plant fitness. Although substantial progress has been achieved in understanding leaf senescence in annual plants, the molecular factors that control leaf senescence in perennial woody plants are largely unknown. Using RNA sequencing, we obtained a high-resolution temporal profile of gene expression during autumn leaf senescence in poplar (Populus tomentosa). Identification of hub transcription factors (TFs) by co-expression network analysis of genes revealed that senescence-associated NAC family TFs (Sen-NAC TFs) regulate autumn leaf senescence. Age-dependent alternative splicing (AS) caused an intron retention (IR) event in the pre-mRNA encoding PtRD26, a NAC-TF. This produced a truncated protein PtRD26IR, which functions as a dominant-negative regulator of senescence by interacting with multiple hub Sen-NAC TFs, thereby repressing their DNA-binding activities. Functional analysis of senescence-associated splicing factors identified two U2 auxiliary factors that are involved in AS of PtRD26IR. Correspondingly, silencing of these factors decreased PtRD26IR transcript abundance and induced early senescence. We propose that an age-dependent increase of IR splice variants derived from Sen-NAC TFs is a regulatory program to fine tune the molecular mechanisms that regulate leaf senescence in trees.
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