ArticleThe Plant cell2021
Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization.
Article in The Plant cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- A practical guide to investigating biomolecular condensates: a comment from the plant community.Science China. Life sciences · 2026Review
- A co-overexpression strategy allows effective protein-protein binding affinities to be assessed as a function of concentration within live cells.bioRxiv : the preprint server for biology · 2026Article
- Dual ubiquitin signalling by SLOMO controls AUX1 activity and turnover during root gravitropism.The EMBO journal · 2026Article
- A Hot-Swappable Genetic Switch: Building an Inducible and Trackable Functional Assay for the Essential Gene MEDIATOR 21.ACS synthetic biology · 2025Article
- Identification of thePlants (Basel, Switzerland) · 2025Article
- ReLo is a simple and rapid colocalization assay to identify and characterize direct protein-protein interactions.Nature communications · 2024Article
- Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants.Nature cell biology · 2024Article
- Phase separation-based visualization of protein-protein interactions and kinase activities in plants.The Plant cell · 2023Article
- The transcription factor AtMYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature.Journal of experimental botany · 2023Article
- From prediction to function: Current practices and challenges towards the functional characterization of type III effectors.Frontiers in microbiology · 2023Review
- Protein-Protein Interactions in Abiotic Stress Signaling: An Overview of Biochemical and Biophysical Methods of Characterization.Methods in molecular biology (Clifton, N.J.) · 2023Review
- The endocytic TPLATE complex internalizes ubiquitinated plasma membrane cargo.Nature plants · 2022Article
- TraB family proteins are components of ER-mitochondrial contact sites and regulate ER-mitochondrial interactions and mitophagy.Nature communications · 2022Article
- Visualization of in vivo protein-protein interactions in plants.Journal of experimental botany · 2022Review
- Conditional and tissue-specific approaches to dissect essential mechanisms in plant development.Current opinion in plant biology · 2022Review
- Overview of methods for characterization and visualization of a protein-protein interaction network in a multi-omics integration context.Frontiers in molecular biosciences · 2022Review
- Knocksideways in plants: an inducible system for in planta visualization of protein interactions.The Plant cell · 2021Article
- Molecular architecture of the endocytic TPLATE complex.Science advances · 2021Article
- Nanobody-Dependent Delocalization of Endocytic Machinery inFrontiers in plant science · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Identifying protein-protein interactions (PPIs) is crucial for understanding biological processes. Many PPI tools are available, yet only some function within the context of a plant cell. Narrowing down even further, only a few tools allow complex multi-protein interactions to be visualized. Here, we present a conditional in vivo PPI tool for plant research that meets these criteria. Knocksideways in plants (KSP) is based on the ability of rapamycin to alter the localization of a bait protein and its interactors via the heterodimerization of FKBP and FRB domains. KSP is inherently free from many limitations of other PPI systems. This in vivo tool does not require spatial proximity of the bait and prey fluorophores and it is compatible with a broad range of fluorophores. KSP is also a conditional tool and therefore the visualization of the proteins in the absence of rapamycin acts as an internal control. We used KSP to confirm previously identified interactions in Nicotiana benthamiana leaf epidermal cells. Furthermore, the scripts that we generated allow the interactions to be quantified at high throughput. Finally, we demonstrate that KSP can easily be used to visualize complex multi-protein interactions. KSP is therefore a versatile tool with unique characteristics and applications that complements other plant PPI methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.