ArticleAngewandte Chemie (International ed. in English)2021
Kinetic Microscale Thermophoresis for Simultaneous Measurement of Binding Affinity and Kinetics.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Discovery and Evaluation of Novel Calenduloside E Derivatives Targeting HSP90β in Ox-LDL-Induced HUVECs Injury.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Macromolecular High-Affinity Binding Probed by Advanced Fluorescence Techniques.Chembiochem : a European journal of chemical biology · 2025Review
- Aptamer Sequence Optimization and Its Application in Food Safety Analysis.Foods (Basel, Switzerland) · 2025Review
- Non-steady state thermometry with optical diffraction tomography.Science advances · 2024Article
- Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins.PloS one · 2024Article
- The Thermodynamic Fingerprints of Ultra-Tight Nanobody-Antigen Binding Probed via Two-Color Single-Molecule Coincidence Detection.International journal of molecular sciences · 2023Article
- An Analysis of Nucleotide-Amyloid Interactions Reveals Selective Binding to Codon-Sized RNA.Journal of the American Chemical Society · 2023Article
- Article
- Kinetic Analysis of Prostate-Specific Antigen Interaction with Monoclonal Antibodies for Development of a Magnetic Immunoassay Based on Nontransparent Fiber Structures.Molecules (Basel, Switzerland) · 2022Article
- Complementary Experimental Methods to Obtain Thermodynamic Parameters of Protein Ligand Systems.International journal of molecular sciences · 2022Article
- Multivalent binding kinetics resolved by fluorescence proximity sensing.Communications biology · 2022Article
- The First Online Capillary Electrophoresis-Microscale Thermophoresis (CE-MST) Method for the Analysis of Dynamic Equilibria-The Determination of the Acidity Constant of Fluorescein Isothiocyanate.Molecules (Basel, Switzerland) · 2022Article
- Microscale Thermophoresis as a Tool to Study Protein Interactions and Their Implication in Human Diseases.International journal of molecular sciences · 2022Review
- AdpA, a developmental regulator, promotes ε-poly-L-lysine biosynthesis in Streptomyces albulus.Microbial cell factories · 2022Article
- The Acid-Base/Deprotonation Equilibrium Can Be Studied with a MicroScale Thermophoresis (MST).Molecules (Basel, Switzerland) · 2022Article
- Kinetic Microscale Thermophoresis for Simultaneous Measurement of Binding Affinity and Kinetics.Angewandte Chemie (International ed. in English) · 2021Article
Corrections and comments
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Authors and funding
3 authors.
Funding
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Abstract
Microscale thermophoresis (MST) is a versatile technique to measure binding affinities of binder-ligand systems, based on the directional movement of molecules in a temperature gradient. We extended MST to measure binding kinetics as well as binding affinity in a single experiment by increasing the thermal dissipation of the sample. The kinetic relaxation fingerprints were derived from the fluorescence changes during thermodynamic re-equilibration of the sample after local heating. Using this method, we measured DNA hybridization on-rates and off-rates in the range 10
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