Evidence map›Paper›PMID 33792795›Full record

ArticleTransgenic research2021

Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus).

Yingqi Huang, William Bugg, Max Bangs, Guyu Qin, David Drescher, Nathan Backenstose, Chia Chen Weng, Yiliu Zhang, Karim Khalil, Sheng Dong and 14 more

Abstract read
PubMed Publisher
In one paragraph

Article in Transgenic research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. CRISPR-Based Transcriptional Activation in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors at 12 institutions in 6 countries.

Yingqi HuangCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
William BuggDepartment of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.
Max BangsDepartment of Biological Sciences, Florida State University, Tallahassee, FL, 32304, USA.
Guyu QinDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
David DrescherMuckleshoot Indian Tribe Department of Fisheries, 39015-A 172nd Ave SE, Auburn, WA, 98092, USA.
Nathan BackenstoseDepartment of Biological Sciences, University At Buffalo, Buffalo, NY, 14260, USA.
Chia Chen WengSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA.
Yiliu ZhangCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Karim KhalilAnatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Sheng DongDepartment of Civil and Environmental Engineering, Auburn University, Auburn, AL, 36849, USA.
Ahmed ElaswadDepartment of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Zhi YeDepartment of Biochemistry, University of Washington, Seattle, WA, 98195-7350, USA.
Cuiyu LuSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA.
Khoi VoDepartment of Aquacultures and Technology, Can Tho Technical Economic College, Can Tho City, Vietnam.
Rhoda Mae SimoraCollege of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao, 5023, Iloilo, Philippines.
Xiaoli MaSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA.
Zachary TaylorSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA.
Yujia YangSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA.
Tao ZhouSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA.
Jingping GuoSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA.
Guillaume SalzeKnipBio, 110 Canal St, Lowell, MA, 01852, USA.
Zhenkui QinMOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
Yi WangBiosystems Engineering Department, Auburn University, Auburn, AL, 36849, USA.
Rex A DunhamSchool of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA. dunhara@auburn.edu.ORCID 0000-0002-6149-6017
Auburn University · USShanghai Ocean University · CNBrigham and Women's Hospital · USCairo University · EGCan Tho University · VNFlorida State University · USOcean University of China · CNSuez Canal University · EGUniversity at Buffalo, State University of New York · USUniversity of Manitoba · CAUniversity of the Philippines Visayas · PHUniversity of Washington · US

Funding

Ocean University of China-uburn University Joint Center 247739
6 · The paper itself

Abstract

Channel catfish (Ictalurus punctatus) is the primary culture species in the US along with its hybrid made with male blue catfish, I. furcatus. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the effectiveness of βactin-D5D for improving n-3 fatty acid production in F1 transgenic channel catfish, as well as examine pleiotropic effects on growth, proximate analysis, disease resistance, and other performance traits. Transgenic F1 channel catfish showed a 33% increase in the relative proportion of n-3 fatty acids coupled with a 15% decrease in n-6 fatty acids and a 17% decrease in n-9 fatty acids when compared to non-transgenic full-siblings (P < 0.01, P < 0.01, P < 0.01). However, while the relative proportion of n-3 fatty acids was achieved, the total amount of fatty acids in the transgenic fish decreased resulting in a reduction of all fatty acids. Insertion of the βactin-D5D transgene into channel catfish also had large effects on the body composition, and growth of channel catfish. Transgenic channel catfish grew faster, were more disease resistant, had higher protein and moisture percentage, but lower fat percentage than full-sib controls. There were sex effects as performance changes were more dramatic and significant in males. The βactin-D5D transgenic channel catfish were also more uniform in their fatty acid composition, growth and other traits.

Indexed as

TransgenesAnimalsAnimals, Genetically ModifiedDelta-5 Fatty Acid DesaturaseFatty AcidsFish ProteinsFlavobacteriumIctaluridaeDelta-5 Fatty Acid DesaturaseFatty AcidsFish ProteinsChannel catfishCulture traitsDesaturaseFatty acidN-3 fatty acidSalmonTransgenic

Identifiers

PMID33792795
OpenAlexW3147549130

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.