ArticleNature communications2021
Connecting structure to function with the recovery of over 1000 high-quality metagenome-assembled genomes from activated sludge using long-read sequencing.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 173 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
173 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The microbial dark matter and "wanted list" in worldwide wastewater treatment plants.Microbiome · 2023Pooled it
- Bioactive molecules unearthed by terabase-scale long-read sequencing of a soil metagenome.Nature biotechnology · 2026Article
- Isolation and characterization of microalgal growth-enhancing bacteria from a wastewater treatment facility.World journal of microbiology & biotechnology · 2026Article
- Article
- Review
- Predicting microbial community responses to disturbance using genome-resolved trait-based life-history strategies.The ISME journal · 2026Article
- Progress in interventions for vaginal microecology.Frontiers in cellular and infection microbiology · 2026Review
- Rethinking metagenome-assembled genome completeness: are we truly recovering complete genomes?Frontiers in microbiology · 2026Article
- A genomic view of Earth's biomes.Nature reviews. Genetics · 2026Review
- Current Understanding of Taxonomy and Ecology of the Phylum Minisyncoccota.Microbes and environments · 2026Review
- Comparative genomic analyses provide new insights into phylogenetic and functional diversification in genusFrontiers in microbiology · 2026Article
- Overlooked siderophore producers favor ammonium oxidation in global wastewater treatment plants.Microbiome · 2025Article
- Metabolic Potential of Candidatus Saccharimonadia Including Rare Lineages in Activated Sludge.Environmental microbiology reports · 2025Article
- Denitrification is a community trait with partial pathways dominating across microbial genomes and biomes.Nature communications · 2025Article
- Automated environmental metagenomics using Oxford nanopore sequencing.BMC genomics · 2025Article
- Article
- Distinct shifts in bacteriophage diversity and abundance during various stages of Gouda-type cheese production.Applied and environmental microbiology · 2025Article
- Halogenation and Dehalogenation Potential of Microorganisms in Yangtze River Waters.Microorganisms · 2025Article
- A Graph Contrastive Learning Method for Enhancing Genome Recovery in Complex Microbial Communities.Entropy (Basel, Switzerland) · 2025Article
- Why Are Long-Read Sequencing Methods Revolutionizing Microbiome Analysis?Microorganisms · 2025Review
113 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microorganisms play crucial roles in water recycling, pollution removal and resource recovery in the wastewater industry. The structure of these microbial communities is increasingly understood based on 16S rRNA amplicon sequencing data. However, such data cannot be linked to functional potential in the absence of high-quality metagenome-assembled genomes (MAGs) for nearly all species. Here, we use long-read and short-read sequencing to recover 1083 high-quality MAGs, including 57 closed circular genomes, from 23 Danish full-scale wastewater treatment plants. The MAGs account for ~30% of the community based on relative abundance, and meet the stringent MIMAG high-quality draft requirements including full-length rRNA genes. We use the information provided by these MAGs in combination with >13 years of 16S rRNA amplicon sequencing data, as well as Raman microspectroscopy and fluorescence in situ hybridisation, to uncover abundant undescribed lineages belonging to important functional groups.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.