ArticleHorticulture research2021
CRISPR/Cas9-mediated mutagenesis of ClBG1 decreased seed size and promoted seed germination in watermelon.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- Molecular Targets and Trait Innovation in CRISPR-Edited Vegetable Crops: An Up-to-Date Review (2020-2026).Plants (Basel, Switzerland) · 2026Review
- Article
- A High-Thousand-Seed-Weight Mutant ofInternational journal of molecular sciences · 2026Article
- Genome-Wide Identification, Characterization, Expression Analysis, and Interacting Protein Prediction of thePlants (Basel, Switzerland) · 2026Article
- Genomic resources and genetic improvement of vital tropical and subtropical fruit crops: current status and prospects.AoB PLANTS · 2025Review
- Identification of candidate genes for watermelon (Molecular breeding : new strategies in plant improvement · 2025Article
- Utilizing CRISPR-based genetic modification for precise control of seed dormancy: progress, obstacles, and potential directions.Molecular biology reports · 2025Review
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- Article
- Telomere-to-telomere Citrullus super-pangenome provides direction for watermelon breeding.Nature genetics · 2024Article
- When Size Matters: New Insights on How Seed Size Can Contribute to the Early Stages of Plant Development.Plants (Basel, Switzerland) · 2024Review
- Natural Allelic Variations ofInternational journal of molecular sciences · 2024Article
- Metabolic, transcriptomic, and genetic analyses of candidate genes for seed size in watermelon.Frontiers in plant science · 2024Article
- Integrated analysis of biparental and natural populations reveals CRIB domain-containing protein underlying seed coat crack trait in watermelon.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Applications of CRISPR/Cas genome editing in economically important fruit crops: recent advances and future directions.Molecular horticulture · 2023Review
- Fine mapping of the major geneFrontiers in plant science · 2023Article
- Watermelon goes viral: introducing a vector for virus-induced gene silencing in cucurbits.Plant physiology · 2022Article
- Transgenic Improvement for Biotic Resistance of Crops.International journal of molecular sciences · 2022Review
- Efficient genetic transformation and CRISPR/Cas9-mediated genome editing of watermelon assisted by genes encoding developmental regulators.Journal of Zhejiang University. Science. B · 2022Article
- Metalliferous habitats and seed microbes affect the seed morphology and reproductive strategy ofPlant and soil · 2022Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Abscisic acid (ABA) is a critical regulator of seed development and germination. β-glucosidases (BGs) have been suggested to be contributors to increased ABA content because they catalyze the hydrolysis of ABA-glucose ester to release free ABA. However, whether BGs are involved in seed development is unclear. In this study, a candidate gene, ClBG1, in watermelon was selected for targeted mutagenesis via the CRISPR/Cas9 system. Seed size and weight were significantly reduced in the Clbg1-mutant watermelon lines, which was mainly attributed to decreased cell number resulting from decreased ABA levels. A transcriptome analysis showed that the expression of 1015 and 1429 unique genes was changed 10 and 18 days after pollination (DAP), respectively. Cytoskeleton- and cell cycle-related genes were enriched in the differentially expressed genes of wild type and Clbg1-mutant lines during seed development. Moreover, the expression of genes in the major signaling pathways of seed size control was also changed. In addition, seed germination was promoted in the Clbg1-mutant lines due to decreased ABA content. These results indicate that ClBG1 may be critical for watermelon seed size regulation and germination mainly through the modulation of ABA content and thereby the transcriptional regulation of cytoskeleton-, cell cycle- and signaling-related genes. Our results lay a foundation for dissecting the molecular mechanisms of controlling watermelon seed size, a key agricultural trait of significant economic importance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.