Evidence map›Paper›PMID 33790234›Full record

ArticleHorticulture research2021

Molecular and physiological characterization of the effects of auxin-enriched rootstock on grafting.

Longmei Zhai, Xiaomin Wang, Dan Tang, Qi Qi, Huseyin Yer, Xiangning Jiang, Zhenhai Han, Richard McAvoy, Wei Li, Yi Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
6.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 46 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Horticulture research · 2025
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Longmei Zhai *Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Xiaomin Wang *Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Dan TangDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Qi QiCollege of Horticulture, China Agricultural University, Beijing, 100193, PR China.
Huseyin YerDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Xiangning JiangNational Engineering Laboratory for Tree Breeding, College of Life Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, PR China.
Zhenhai HanCollege of Horticulture, China Agricultural University, Beijing, 100193, PR China.
Richard McAvoyDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA.
Wei LiDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA. liwei0522898@163.com.
Yi LiDepartment of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT, 06269, USA. yi.li@uconn.edu.
University of Connecticut · USChina Agricultural University · CNBeijing Forestry University · CNInstitute of Botany · CN

Funding

Citrus Research and Development Foundation (CRDF) Projects Li-749 and 16-001
6 · The paper itself

Abstract

Grafting is a highly useful technique, and its success largely depends on graft union formation. In this study, we found that root-specific expression of the auxin biosynthetic gene iaaM in tobacco, when used as rootstock, resulted in more rapid callus formation and faster graft healing. However, overexpression of the auxin-inactivating iaaL gene in rootstocks delayed graft healing. We observed increased endogenous auxin levels and auxin-responsive DR5::GUS expression in scions of WT/iaaM grafts compared with those found in WT/WT grafts, which suggested that auxin is transported upward from rootstock to scion tissues. A transcriptome analysis showed that auxin enhanced graft union formation through increases in the expression of genes involved in graft healing in both rootstock and scion tissues. We also observed that the ethylene biosynthetic gene ACS1 and the ethylene-responsive gene ERF5 were upregulated in both scions and rootstocks of the WT/iaaM grafts. Furthermore, exogenous applications of the ethylene precursor ACC to the junction of WT/WT grafts promoted graft union formation, whereas application of the ethylene biosynthesis inhibitor AVG delayed graft healing in WT/WT grafts, and the observed delay was less pronounced in the WT/iaaM grafts. These results demonstrated that elevated auxin levels in the iaaM rootstock in combination with the increased auxin levels in scions caused by upward transport/diffusion enhanced graft union formation and that ethylene was partially responsible for the effects of auxin on grafting. Our findings showed that grafting success can be enhanced by increasing the auxin levels in rootstocks using transgenic or gene-editing techniques.

Identifiers

PMID33790234
PMCPMC8012700
OpenAlexW3140137956

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.