ArticlemSphere2021
Stability of SARS-CoV-2 Spike G614 Variant Surpasses That of the D614 Variant after Cold Storage.
Article in mSphere, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- A Qualitative Framework for Ranking the Relative Likelihood of SARS-CoV-2 Transmission Between People and Felids at Zoological Interfaces.Animals : an open access journal from MDPI · 2026Article
- Ancestral Lineage of SARS-CoV-2 Is More Stable in Human Biological Fluids than Alpha, Beta, and Omicron Variants of Concern.Microbiology spectrum · 2023Article
- Nature-Inspired Surface Structures Design for Antimicrobial Applications.International journal of molecular sciences · 2023Review
- Rural populations facilitated early SARS-CoV-2 evolution and transmission in Missouri, USA.Npj viruses · 2023Article
- Review
- Database of SARS-CoV-2 and coronaviruses kinetics relevant for assessing persistence in food processing plants.Scientific data · 2022Article
- Disinfection and decontamination in the context of SARS-CoV-2-specific data.Journal of medical virology · 2022Review
- Ancestral lineage of SARS-CoV-2 is more stable in human biological fluids than Alpha, Beta and Omicron variants of concern.bioRxiv : the preprint server for biology · 2022Article
- Inactivation of porcine epidemic diarrhea virus with electron beam irradiation under cold chain conditions.Environmental technology & innovation · 2022Article
- Molecular Virology of SARS-CoV-2 and Related Coronaviruses.Microbiology and molecular biology reviews : MMBR · 2022Review
- Review
- COVID-19 Genetic Variants and Their Potential Impact in Vaccine Development.Microorganisms · 2022Review
- Comprehensive Risk Pathway of the Qualitative Likelihood of Human Exposure to Severe Acute Respiratory Syndrome Coronavirus 2 from the Food Chain.Journal of food protection · 2022Article
- Cold chain logistics: a possible mode of SARS-CoV-2 transmission?BMJ (Clinical research ed.) · 2021Article
- Sensitivity of two SARS-CoV-2 variants with spike protein mutations to neutralising antibodies.Virus genes · 2021Article
- Article
- Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) carrying the D614G mutation on the spike protein is the predominant circulating variant and is associated with enhanced infectivity. However, whether this dominant variant can potentially spread through the cold chain and whether the spike protein affects virus stability after cold storage remain unclear. To compare the infectivity of two SARS-CoV-2 variants, namely, SARS-CoV-2 variants with spike protein with the D614 mutation (S-D614) and G614 mutation (S-G614), after different periods of refrigeration (4°C) and freezing (-20°C). We also determined the integrity of the viral RNA and the ability of the spike protein to bind angiotensin-converting enzyme 2 (ACE2) after storage at these conditions. The results showed that SARS-CoV-2 was more stable and infectious after storage at -20°C than at 4°C. Particularly, the S-G614 variant was found to be more stable than the S-D614 variant. The spike protein of the S-G614 variant had better binding ability with the ACE2 receptor than that of the S-D614 variant after storage at -20°C for up to 30 days. Our findings revealed that SARS-CoV-2 remains stable and infectious after refrigeration or freezing, and their stability and infectivity up to 30 days depends on the spike variant. Stability and infectivity are related to each other, and the higher stability of S-G614 compared to that of S-D614 may contribute to rapid viral spread of the S-G614 variant.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.