Evidence map›Paper›PMID 33787948›Full record

ReviewEuropean biophysics journal : EBJ2021

Selected tools to visualize membrane interactions.

Tobias Grothe, Julia Nowak, Reinhard Jahn, Peter Jomo Walla

Open access · hybridAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in European biophysics journal : EBJ, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.2field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Tobias GrotheLaboratory of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, 37077, Göttingen, Germany.
Julia NowakDepartment of Biophysical Chemistry, Institute for Physical and Theoretical Chemistry, University of Braunschweig, 38106, Braunschweig, Germany.
Reinhard JahnLaboratory of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, 37077, Göttingen, Germany.
Peter Jomo WallaLaboratory of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, 37077, Göttingen, Germany. p.walla@tu-braunschweig.de.ORCID http://orcid.org/0000-0001-6956-5569
Max Planck Institute for Biophysical Chemistry · DETechnische Universität Braunschweig · DE

Funding

Deutsche Forschungsgemeinschaft SFB 803
6 · The paper itself

Abstract

In the past decade, we developed various fluorescence-based methods for monitoring membrane fusion, membrane docking, distances between membranes, and membrane curvature. These tools were mainly developed using liposomes as model systems, which allows for the dissection of specific interactions mediated by, for example, fusion proteins. Here, we provide an overview of these methods, including two-photon fluorescence cross-correlation spectroscopy and intramembrane Förster energy transfer, with asymmetric labelling of inner and outer membrane leaflets and the calibrated use of transmembrane energy transfer to determine membrane distances below 10 nm. We discuss their application range and their limitations using examples from our work on protein-mediated vesicle docking and fusion.

Indexed as

Cell MembraneFluorescence Resonance Energy TransferMembrane FusionFluorescence cross-correlation spectroscopyFluorescence lifetime analysisLiposomesMembrane fusion intermediatesSynaptic proteins

Identifiers

PMID33787948
PMCPMC8071796
OpenAlexW3152307163

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.