Evidence map›Paper›PMID 33786783›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Global Analyses to Identify Direct Transcriptional Targets of p53.

Matthew D Galbraith, Zdenek Andrysik, Kelly D Sullivan, Joaquín M Espinosa

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.1field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Matthew D GalbraithDepartment of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Zdenek AndrysikDepartment of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Kelly D SullivanLinda Crnic Institute for Down Syndrome, School of Medicine, University of Colorado Anschutz Medical Campus, Aurora, CO, USA.
Joaquín M EspinosaDepartment of Pharmacology, University of Colorado Anschutz Medical Campus, Aurora, CO, USA. joaquin.espinosa@cuanschutz.edu.
University of Colorado Anschutz Medical Campus · US

Funding

Stress- and Promoter-Specific Mechanisms of Transcritpional Activation by p53R01CA117907 · NCI · UNIVERSITY OF COLORADO DENVER · PI ESPINOSA, JOAQUIN M. · 2006 to 2022
$4.4M
Howard Hughes Medical InstituteNCI NIH HHS R01 CA117907
6 · The paper itself

Abstract

The transcription factor p53 controls a gene expression program with pleiotropic effects on cell biology including cell cycle arrest and apoptosis. Identifying direct p53 target genes within this network and determining how they influence cell fate decisions downstream of p53 activation is a prerequisite for designing therapeutic approaches that target p53 to effectively kill cancer cells. Here we describe a comprehensive multi-omics approach for identifying genes that are direct transcriptional targets of p53. We provide detailed procedures for measuring global RNA polymerase activity, defining p53 binding sites across the genome, and quantifying changes in steady-state mRNA in response to p53 activation.

Indexed as

TranscriptomeAnimalsCell LineChromatin Immunoprecipitation SequencingGenomicsHumansRNA-SeqTranscriptional ActivationTumor Suppressor Protein p53Tumor Suppressor Protein p53ChIP-seqGene ExpressionGRO-seqNutlinp53RNA-seqSequencingTranscriptionTranscriptome

Identifiers

PMID33786783
OpenAlexW3147515049

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.