Evidence map›Paper›PMID 33786461›Full record

ArticleSTAR protocols2021

CRISPR-Cas9 strategies to insert MS2 stem-loops into endogenous loci in

Caroline Hoppe, Hilary L Ashe

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Navigating the Hurdles of Intra-Articular AAV Gene Therapy.International journal of molecular sciences · 2025
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Expanding Gene-Editing Potential in Crop Improvement with Pangenomes.International journal of molecular sciences · 2022
    Review
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Caroline HoppeFaculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.
Hilary L AsheFaculty of Biology, Medicine and Health, University of Manchester, Manchester M13 9PT, UK.

Funding

Wellcome TrustWellcome Trust 204832/Z/16/Z
6 · The paper itself

Abstract

CRISPR-Cas9 genome editing has transformed biology by enabling site-specific genome modifications to be simply engineered. Here, we describe two CRISPR-Cas9 approaches to introduce MS2 stem-loop sequences into endogenous gene loci in

Indexed as

AnimalsCRISPR-Cas SystemsDrosophilaGene EditingGene Expression ProfilingGenomeInverted Repeat SequencesRNA, MessengerRNA, MessengerCRISPRGeneticsModel organismsMolecular biology

Identifiers

PMID33786461
PMCPMC7988240

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.