Evidence map›Paper›PMID 33786459›Full record

ArticleSTAR protocols2021

Functional proteomics protocol for the identification of interaction partners in

Syed Nabeel-Shah, Jyoti Garg, Pata-Eting Kougnassoukou Tchara, Ronald E Pearlman, Jean-Philippe Lambert, Jeffrey Fillingham

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Syed Nabeel-ShahDepartment of Chemistry and Biology, Ryerson University, 350 Victoria Street, Toronto, ON M5B 2K3, Canada.
Jyoti GargDepartment of Chemistry and Biology, Ryerson University, 350 Victoria Street, Toronto, ON M5B 2K3, Canada.
Pata-Eting Kougnassoukou TcharaDepartment of Molecular Medicine, Cancer Research Center, Big Data Research Center, Université Laval, Quebec City, QC, Canada.
Ronald E PearlmanDepartment of Biology, York University, 4700 Keele Street, Toronto, ON, M3J 1P3, Canada.
Jean-Philippe LambertDepartment of Molecular Medicine, Cancer Research Center, Big Data Research Center, Université Laval, Quebec City, QC, Canada.
Jeffrey FillinghamDepartment of Chemistry and Biology, Ryerson University, 350 Victoria Street, Toronto, ON M5B 2K3, Canada.
Centre hospitalier universitaire de Québec · CAYork University · CAToronto Metropolitan University · CAUniversity of Toronto · CA

Funding

CIHR MOP13347
6 · The paper itself

Abstract

We describe an optimized protocol for one-step affinity purification of FZZ-tagged proteins followed by mass spectrometry analysis for the identification of protein-protein interactions in the ciliate protozoan

Indexed as

ProteomicsProtozoan ProteinsTetrahymena thermophilaProtozoan ProteinsMass spectrometryModel organismsMolecular biologyProtein biochemistryProtein expression and purificationProteomics

Identifiers

PMID33786459
PMCPMC7988224
OpenAlexW3134857564

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.