ArticleAIP advances2021
Continuous and discrete phasor analysis of binned or time-gated periodic decays.
Article in AIP advances, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera.Light, science & applications · 2025Article
- Fluorescence Lifetime Endoscopy with a Nanosecond Time-Gated CAPS Camera with IRF-Free Deep Learning Method.Sensors (Basel, Switzerland) · 2025Article
- Real-time wide-field fluorescence lifetime imaging via single-snapshot acquisition for biomedical applications.PhotoniX · 2025Article
- Deciphering amyloid fibril molecular maturation through FLIM-phasor analysis of thioflavin T.Biophysical reports · 2024Article
- Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles.Biophysical reports · 2023Article
- In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET.Biophysical reports · 2023Article
- Article
- FLUTE: A Python GUI for interactive phasor analysis of FLIM data.Biological imaging · 2023Article
- Article
- Linear Combination Properties of the Phasor Space in Fluorescence Imaging.Sensors (Basel, Switzerland) · 2022Review
- An overview of continuous and discrete phasor analysis of binned or time-gated periodic decays.Proceedings of SPIE--the International Society for Optical Engineering · 2021Article
- Article
- NIR Fluorescence lifetime macroscopic imaging with a time-gated SPAD camera.Proceedings of SPIE--the International Society for Optical EngineeringArticle
- NIR Fluorescence lifetime macroscopic imaging with a novel time-gated SPAD camera.Proceedings of SPIE--the International Society for Optical EngineeringArticle
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
The time-resolved analysis of periodically excited luminescence decays by the phasor method in the presence of time-gating or binning is revisited. Analytical expressions for discrete configurations of square gates are derived, and the locus of the phasors of such modified periodic single-exponential decays is compared to the canonical universal semicircle. The effects of instrument response function offset, decay truncation, and gate shape are also discussed. Finally, modified expressions for the phase and modulus lifetimes are provided for some simple cases. A discussion of a modified phasor calibration approach is presented, and an illustration of the new concepts with examples from the literature concludes this work.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.