Evidence map›Paper›PMID 33785826›Full record

ArticleScientific reports2021

Delineating proinflammatory microenvironmental signals by ex vivo modeling of the immature intestinal stroma.

Mari Ichinose, Nobumi Suzuki, Tongtong Wang, Josephine A Wright, Tamsin R M Lannagan, Laura Vrbanac, Hiroki Kobayashi, Krystyna Gieniec, Jia Q Ng, Souzaburo Ihara and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 2 countries.

Mari Ichinose *School of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Nobumi Suzuki *School of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Tongtong WangSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Josephine A WrightSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Tamsin R M LannaganSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Laura VrbanacSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Hiroki KobayashiSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Krystyna GieniecSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Jia Q NgSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Souzaburo IharaDepartment of Gastroenterology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Chris MavrangelosSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Yoku HayakawaDepartment of Gastroenterology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
Patrick HughesSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia.
Daniel L WorthleySouth Australian Health and Medical Research Institute, Adelaide, SA, 5000, Australia.
Susan L WoodsSchool of Medicine, University of Adelaide, Adelaide, SA, 5000, Australia. susan.woods@adelaide.edu.au.
University of Adelaide · AUSouth Australian Health and Medical Research Institute · AUThe University of Tokyo · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The intestinal stroma provides an important microenvironment for immune cell activation. The perturbation of this tightly regulated process can lead to excessive inflammation. We know that upregulated Toll-like receptor 4 (TLR4) in the intestinal epithelium plays a key role in the inflammatory condition of preterm infants, such as necrotizing enterocolitis (NEC). However, the surrounding stromal contribution to excessive inflammation in the pre-term setting awaits careful dissection. Ex vivo co-culture of embryonic day 14.5 (E14.5) or adult murine intestinal stromal cells with exogenous monocytes was undertaken. We also performed mRNAseq analysis of embryonic and adult stromal cells treated with vehicle control or lipopolysaccharide (LPS), followed by pathway and network analyses of differentially regulated transcripts. Cell characteristics were compared using flow cytometry and pHrodo red phagocytic stain, candidate gene analysis was performed via siRNA knockdown and gene expression measured by qPCR and ELISA. Embryonic stromal cells promote the differentiation of co-cultured monocytes to CD11b

Indexed as

AnimalsCells, CulturedCoculture TechniquesEnterocolitis, NecrotizingGene Regulatory NetworksHumansInfant, NewbornInfant, PrematureInflammationIntestinesMiceMice, Inbred C57BLMonocytesStromal CellsTranscriptome

Identifiers

PMID33785826
PMCPMC8010037
OpenAlexW3147943907

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.