ArticleThe Journal of biological chemistry
Biophysical analysis of the plant-specific GIPC sphingolipids reveals multiple modes of membrane regulation.
Article in The Journal of biological chemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Advanced lipidomic techniques for high-throughput profiling of complex sphingolipids in plant tissues.Journal of plant research · 2026Article
- Plant-derived extracellular vesicles for drug delivery: current and future.Regenerative biomaterials · 2026Review
- Deciphering the Sodium Sensing Mechanisms in Glycophytes and Halophytes.Plant, cell & environment · 2025Review
- Plasticity of the cytotoxic Nep1-like protein enables promiscuity in binding to its lipid receptor glycosylinositol phosphorylceramides.Science advances · 2025Article
- Chemical Synthesis and Structural Determination of the Inositol Glycan Head of Plant Sphingolipid GIPC in Brassicaceae.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Interaction of an Oomycete Nep1-like Cytolysin with Natural and Plant Cell-Mimicking Membranes.The Journal of membrane biology · 2025Article
- Automated mass spectrometry-based profiling of multi-glycosylated glycosyl inositol phospho ceramides (GIPC) reveals specific series GIPC rearrangements during barley grain development and heat stress response.The Plant journal : for cell and molecular biology · 2025Article
- Identification of INOSITOL PHOSPHORYLCERAMIDE SYNTHASE 2 (IPCS2) as a new rate-limiting component in Arabidopsis pathogen entry control.The Plant journal : for cell and molecular biology · 2025Article
- A fungal phospholipase C involved in the degradation of plant glycosylinositol phosphorylceramides during Arabidopsis/Botrytis interaction.Communications biology · 2024Article
- Intracellular sphingolipid sorting drives membrane phase separation in the yeast vacuole.The Journal of biological chemistry · 2024Article
- Structural determinants of REMORIN nanodomain formation in anionic membranes.Biophysical journal · 2023Article
- Fatty Acid 2-Hydroxylase and 2-Hydroxylated Sphingolipids: Metabolism and Function in Health and Diseases.International journal of molecular sciences · 2023Review
- Lipid Rafts and Plant Gravisensitivity.Life (Basel, Switzerland) · 2022Review
- A combination of plasma membrane sterol biosynthesis and autophagy is required for shade-induced hypocotyl elongation.Nature communications · 2022Article
- Sphingolipids are involved in insect egg-induced cell death in Arabidopsis.Plant physiology · 2022Article
- Cytotoxic activity of Nep1-like proteins on monocots.The New phytologist · 2022Article
- Sphingolipids with 2-hydroxy fatty acids aid in plasma membrane nanodomain organization and oxidative burst.Plant physiology · 2022Article
- Diversity in sphingolipid metabolism across land plants.Journal of experimental botany · 2022Review
- Novel Chemical and Biological Insights of Inositol Derivatives in Mediterranean Plants.Molecules (Basel, Switzerland) · 2022Review
- Sphingolipids mediate polar sorting of PIN2 through phosphoinositide consumption at the trans-Golgi network.Nature communications · 2021Article
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Authors and funding
19 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant plasma membrane (PM) is an essential barrier between the cell and the external environment, controlling signal perception and transmission. It consists of an asymmetrical lipid bilayer made up of three different lipid classes: sphingolipids, sterols, and phospholipids. The glycosyl inositol phosphoryl ceramides (GIPCs), representing up to 40% of total sphingolipids, are assumed to be almost exclusively in the outer leaflet of the PM. However, their biological role and properties are poorly defined. In this study, we investigated the role of GIPCs in membrane organization. Because GIPCs are not commercially available, we developed a protocol to extract and isolate GIPC-enriched fractions from eudicots (cauliflower and tobacco) and monocots (leek and rice). Lipidomic analysis confirmed the presence of trihydroxylated long chain bases and 2-hydroxylated very long-chain fatty acids up to 26 carbon atoms. The glycan head groups of the GIPCs from monocots and dicots were analyzed by gas chromatograph-mass spectrometry, revealing different sugar moieties. Multiple biophysics tools, namely Langmuir monolayer, ζ-Potential, light scattering, neutron reflectivity, solid state 2H-NMR, and molecular modeling, were used to investigate the physical properties of the GIPCs, as well as their interaction with free and conjugated phytosterols. We showed that GIPCs increase the thickness and electronegativity of model membranes, interact differentially with the different phytosterols species, and regulate the gel-to-fluid phase transition during temperature variations. These results unveil the multiple roles played by GIPCs in the plant PM.
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