ReviewCurrent opinion in chemical biology2021
Systems for in vivo hypermutation: a quest for scale and depth in directed evolution.
Review in Current opinion in chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed.
- Harnessing the central dogma: Enzyme-driven gene diversification strategies for in cellulo continuous directed evolution.Fundamental research · 2026Review
- Exploring the Evolutionary Landscape with Targeted In Vivo Hypermutations.Biomedicines · 2026Review
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Continuous targeted hypermutation with a tunable mutation window.Nature communications · 2025Article
- Article
- T7 RNA polymerase-guided base editor for accelerated continuous evolution inSynthetic and systems biotechnology · 2025Article
- A Golden Gate compatible system for continuous directed evolution inSynthetic biology (Oxford, England) · 2025Article
- Enzyme Engineering: Performance Optimization, Novel Sources, and Applications in the Food Industry.Foods (Basel, Switzerland) · 2024Review
- Dual genetic level modification engineering accelerate genome evolution of Corynebacterium glutamicum.Nucleic acids research · 2024Article
- Automated in vivo enzyme engineering accelerates biocatalyst optimization.Nature communications · 2024Review
- Ultrahigh-throughput screening-assisted in vivo directed evolution for enzyme engineering.Biotechnology for biofuels and bioproducts · 2024Article
- Engineered bacterial orthogonal DNA replication system for continuous evolution.Nature chemical biology · 2023Article
- Steering and controlling evolution - from bioengineering to fighting pathogens.Nature reviews. Genetics · 2023Review
- Evolution and synthetic biology.Current opinion in microbiology · 2023Review
- Adapting enzymes to improve their functionality in plants: why and how.Biochemical Society transactions · 2023Review
- A dual gene-specific mutator system installs all transition mutations at similar frequencies in vivo.Nucleic acids research · 2023Article
- Directed Evolution of Aerotolerance in Sulfide-Dependent Thiazole Synthases.ACS synthetic biology · 2023Article
- Continuous Directed Evolution of a Feedback-ResistantACS synthetic biology · 2023Article
- The G285S mutation in nsP1 is sufficient to render Sindbis virus as a stable vector for gene delivery.Frontiers in microbiology · 2023Article
- Using continuous directed evolution to improve enzymes for plant applications.Plant physiology · 2022Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Traditional approaches to the directed evolution of genes of interest (GOIs) place constraints on the scale of experimentation and depth of evolutionary search reasonably achieved. Engineered genetic systems that dramatically elevate the mutation of target GOIs in vivo relieve these constraints by enabling continuous evolution, affording new strategies in the exploration of sequence space and fitness landscapes for GOIs. We describe various in vivo hypermutation systems for continuous evolution, discuss how different architectures for in vivo hypermutation facilitate evolutionary search scale and depth in their application to problems in protein evolution and engineering, and outline future opportunities for the field.
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