ArticleProceedings of the National Academy of Sciences of the United States of America2021
Turn-on mode diarylethenes for bioconjugation and fluorescence microscopy of cellular structures.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 64 citations in OpenAlex.
- Dihydrobenzofuro[3,2-Chemical science · 2026Article
- Diarylethene-Based Photoswitches in Nanomaterial Hybrids: From Molecular Architecture to Device Amalgamation.Chemistry, an Asian journal · 2026Review
- Fluorescent Diarylethenes With Polar Groups: Synthesis, Spectra, and Optical Microscopy Applications.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Sulfhydryl-functionalized diarylethenes: synthesis, photoswitching, and fluorescent properties.RSC advances · 2026Article
- Turn-on type fluorescent photochromism of a diarylmaleimide-S,S,S',S'-tetraoxide.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- Photoswitchable Fluorescence of Peptide-Based Hemipiperazines Inside of Living Cells.Journal of the American Chemical Society · 2025Article
- Visible-Light-Driven Fluorescence Turn-on Photoswitches With Near Quantitative Photocyclization Yield.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Supramolecular Synthesis of Dithienylethene-Albumin Complexes for Enhanced Photoswitching In Photoacoustic Imaging-Guided Near-Infrared Photothermal Therapy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Near-infrared two-photon absorption and excited state dynamics of a fluorescent diarylethene derivative.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- Bioorthogonal Reactions in Bioimaging.Topics in current chemistry (Cham) · 2024Review
- No-wash fluorogenic labeling of proteins for reversible photoswitching in live cells.Chemical science · 2024Article
- 2,3-Diarylmaleate salts as a versatile class of diarylethenes with a full spectrum of photoactivity in water.Chemical science · 2023Article
- Bioorthogonal Caging-Group-Free Photoactivatable Probes for Minimal-Linkage-Error Nanoscopy.ACS central science · 2023Article
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Hemipiperazines as peptide-derived molecular photoswitches with low-nanomolar cytotoxicity.Nature communications · 2022Article
- Cleavable Linker Incorporation into a Synthetic Dye-Nanobody-Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy.Chembiochem : a European journal of chemical biology · 2022Article
- Green-/NIR-light-controlled rapid photochromism featuring reversible thermally activated delayed fluorescence and photoelectronic switching.Chemical science · 2022Article
- Supramolecular Complex of Photochromic Diarylethene and Cucurbit[7]uril: Fluorescent Photoswitching System for Biolabeling and Imaging.Journal of the American Chemical Society · 2022Article
- A DNA-Based Two-Component Excitonic Switch Utilizing High-Performance Diarylethenes.Angewandte Chemie (International ed. in English) · 2022Article
- Development of Red-Shifted and Fluorogenic Nucleoside and Oligonucleotide Diarylethene Photoswitches.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of photoswitchable fluorescent diarylethenes (fDAEs) as protein labels in fluorescence microscopy and nanoscopy has been limited by labeling inhomogeneity and the need for ultraviolet light for fluorescence activation (on-switching). To overcome these drawbacks, we prepared "turn-on mode" fDAEs featuring thienyl substituents, multiple polar residues, and a reactive maleimide group in the core structure. Conjugates with antibodies and nanobodies displayed complete on-switching and excitation with violet (405 nm) and yellow-green (<565 nm) light, respectively. Besides, they afforded high signal-to-noise ratios and low unspecific labeling in fluorescence imaging. Irradiation with visible light at 532 nm or 561 nm led to transient on-off switching ("blinking") of the fDAEs of double-labeled nanobodies so that nanoscale superresolution images were readily attained through switching and localization of individual fluorophores.
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