Evidence map›Paper›PMID 33781321›Full record

ReviewBiotechnology for biofuels2021

Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.

Brian T DeVree, Lisa M Steiner, Sylwia Głazowska, Felix Ruhnow, Klaus Herburger, Staffan Persson, Jozef Mravec

Abstract readReview
In one paragraph

Review in Biotechnology for biofuels, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Chemical Fingerprint ImagingAnalytical chemistry · 2025
    Article
  7. Article
  8. Review
  9. Review
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Cell wall dynamics: novel tools and research questions.Journal of experimental botany · 2023
    Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Brian T DeVree *Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.ORCID http://orcid.org/0000-0003-0582-8125
Lisa M Steiner *Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.ORCID http://orcid.org/0000-0002-6868-0423
Sylwia GłazowskaDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.ORCID http://orcid.org/0000-0002-8257-4432
Felix RuhnowDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.ORCID http://orcid.org/0000-0002-0856-5511
Klaus HerburgerDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark.ORCID http://orcid.org/0000-0001-8014-0844
Staffan PerssonDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark. staffan.persson@plen.ku.dk.ORCID http://orcid.org/0000-0002-6377-5132
Jozef MravecDepartment of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark. mravec@plen.ku.dk.ORCID http://orcid.org/0000-0003-0331-3304

Funding

Australian Research Council DP190101941Australian Research Council FT160100218Det Frie Forskningsråd 272-07-0152Novo Nordisk Fonden NNF19OC0056076Villum Fonden 00023089Villum Fonden 17489Villum Fonden 25905
6 · The paper itself

Abstract

Plant cell wall-derived biomass serves as a renewable source of energy and materials with increasing importance. The cell walls are biomacromolecular assemblies defined by a fine arrangement of different classes of polysaccharides, proteoglycans, and aromatic polymers and are one of the most complex structures in Nature. One of the most challenging tasks of cell biology and biomass biotechnology research is to image the structure and organization of this complex matrix, as well as to visualize the compartmentalized, multiplayer biosynthetic machineries that build the elaborate cell wall architecture. Better knowledge of the plant cells, cell walls, and whole tissue is essential for bioengineering efforts and for designing efficient strategies of industrial deconstruction of the cell wall-derived biomass and its saccharification. Cell wall-directed molecular probes and analysis by light microscopy, which is capable of imaging with a high level of specificity, little sample processing, and often in real time, are important tools to understand cell wall assemblies. This review provides a comprehensive overview about the possibilities for fluorescence label-based imaging techniques and a variety of probing methods, discussing both well-established and emerging tools. Examples of applications of these tools are provided. We also list and discuss the advantages and limitations of the methods. Specifically, we elaborate on what are the most important considerations when applying a particular technique for plants, the potential for future development, and how the plant cell wall field might be inspired by advances in the biomedical and general cell biology fields.

Indexed as

Anti-glycan antibodiesAptamersCarbohydrate-binding modulesCell wall probesClick chemistryFluorescence microscopyGenetic probesLambodiesLive cell imagingNanobodiesPlant cell wall architectureSuperresolution microscopy

Identifiers

PMID33781321
PMCPMC8008654

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.