ArticlePlant methods2021
Isotope ratio-based quantification of carbon assimilation highlights the role of plastidial isoprenoid precursor availability in photosynthesis.
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Chorismate and isochorismate turnover reveal hidden dynamics of aromatic metabolism in Arabidopsis leaves.The Plant cell · 2026Article
- Assessing and avoiding C isotopic contamination artefacts in mesocosm-scalePlant methods · 2025Article
- Rubisco supplies pyruvate for the 2-C-methyl-D-erythritol-4-phosphate pathway.Nature plants · 2024Article
- Combination of long-termPlant methods · 2022Article
- An Arabidopsis GCMS chemical ionization technique to quantify adaptive responses in central metabolism.Plant physiology · 2022Article
- Design and fabrication of an improved dynamic flow cuvette forPlant methods · 2022Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
Abstract
backgroundWe report a method to estimate carbon assimilation based on isotope ratio-mass spectrometry (IRMS) of
resultsIRMS-based carbon assimilation values from plants labeled at different light intensities were compared to those obtained by gas exchange, and the two methods yielded similar values. Using this method, we observed a strong correlation between
conclusionsThis method provides an alternative to gas exchange that offers several advantages: resilience to differences in leaf overlap, measurements based on tissue mass rather than leaf surface area, and compatibility with mutant Arabidopsis lines which are not amenable to gas exchange measurements due to low biomass and limited leaf surface area. It is suitable for screening large numbers of replicates simultaneously as well as post-hoc analysis of previously labeled plant tissue and is complementary to downstream detection of isotopic label in targeted metabolite pools.
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