ArticleBiosensors & bioelectronics2021
Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests.
Article in Biosensors & bioelectronics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Biophotonics point-of-care diagnostics in low-resource settings: a South African perspective.Journal of biomedical optics · 2026Review
- Hybridization-Based Electrochemical Biosensors for RNA Detection: Key Concepts and Insights.Sensors and actuators. B, Chemical · 2026Article
- SIMPLE: A One-Pot RPA-Cas12b Method for Single Nucleotide Variations Identification with an Expanded Scope and Clinical Applications.Phenomics (Cham, Switzerland) · 2025Article
- MXene-Based Electrochemical Biosensors: Advancing Detection Strategies for Biosensing (2020-2024).Biosensors · 2025Review
- Integrated CRISPR-Cas12a and RAA one-pot visual strategy for the rapid identification ofFrontiers in cellular and infection microbiology · 2025Article
- Nano-modified biosensors for detection of pathogenic diseases: The prospect of smart, multiplex and point-of-care testing.ADMET & DMPK · 2025Review
- Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification.Molecules (Basel, Switzerland) · 2024Review
- One-pot electrochemical detection of foodborne pathogen based on in situ nucleic acid amplification and wash-free assay.Mikrochimica acta · 2024Article
- Sensitive, Semiquantitative, and Portable Nucleic Acid Detection of Rabies Virus Using a Personal Glucose Meter.ACS omega · 2024Article
- Dual-Mode Graphene Field-Effect Transistor Biosensor with Isothermal Nucleic Acid Amplification.Biosensors · 2024Article
- Low-Cost Biosensor Technologies for Rapid Detection of COVID-19 and Future Pandemics.ACS nano · 2024Review
- On-site airborne pathogen detection for infection risk mitigation.Chemical Society reviews · 2023Review
- Development of non-electrically controlled SalivaDirect LAMP (NEC-SD-LAMP), a new nonelectrical infectious disease testing method.Scientific reports · 2023Article
- Insight of smart biosensors for COVID-19: A review.Luminescence : the journal of biological and chemical luminescence · 2023Review
- Recombinase Polymerase Amplification Assay with Lateral Flow Strips for Rapid Detection of Candidiasis Due to Candida parapsilosis.Current microbiology · 2023Article
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- Recent Advances in Molecular and Immunological Diagnostic Platform for Virus Detection: A Review.Biosensors · 2023Review
- Ultrasensitive and amplification-free detection of SARS-CoV-2 RNA using an electrochemical biosensor powered by CRISPR/Cas13a.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Peptide-based direct electrochemical detection of receptor binding domains of SARS-CoV-2 spike protein in pristine samples.Sensors and actuators. B, Chemical · 2023Article
- Advancement in COVID-19 detection using nanomaterial-based biosensors.Exploration (Beijing, China) · 2023Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report an electrochemical biosensor combined with recombinase polymerase amplification (RPA) for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2. The electrochemical biosensor based on a multi-microelectrode array allows the detection of multiple target genes by differential pulse voltammetry. The RPA reaction involves hybridization of the RPA amplicon with thiol-modified primers immobilized on the working electrodes, which leads to a reduction of current density as amplicons accumulate. The assay results in shorter "sample-to-answer" times than conventional PCR without expensive thermo-cycling equipment. The limits of detection are about 0.972 fg/μL (RdRP gene) and 3.925 fg/μL (N gene), which are slightly lower than or comparable to that of RPA assay results obtained by gel electrophoresis without post-amplification purification. The combination of electrochemical biosensors and the RPA assay is a rapid, sensitive, and convenient platform that can be potentially used as a point-of-care test for the diagnosis of COVID-19.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.