ArticlePlant biotechnology journal2021
Metabolic engineering of astaxanthin-rich maize and its use in the production of biofortified eggs.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 30 citations in OpenAlex.
- Developing high-value-added cotton via rebuilding astaxanthin biosynthesis pathway.Plant biotechnology journal · 2025Article
- Oilseed-based metabolic engineering of astaxanthin and related ketocarotenoids using a plant-derived pathway: Lab-to-field-to-application.Plant biotechnology journal · 2025Article
- Breeding triple-advantage cottonseed with higher yield, enhanced nutrition, and reduced toxicity by redirecting terpenoid metabolism to astaxanthin.Plant biotechnology journal · 2025Article
- Agricultural biotechnology in China: product development, commercialization, and perspectives.aBIOTECH · 2025Review
- Multi-Omics Analysis Provides Insights into a Mosaic-Leaf Phenotype of Astaxanthin-Producing Tobacco.Plants (Basel, Switzerland) · 2025Article
- Identification and functional characterization of bidirectional gene pairs and their intergenic regions in cotton.BMC plant biology · 2024Article
- Recent Advances in Astaxanthin as an Antioxidant in Food Applications.Antioxidants (Basel, Switzerland) · 2024Review
- Review
- Deposition and enrichment of carotenoids in livestock products: An overview.Food chemistry: X · 2024Review
- From qualitative to quantitative: the state of the art and challenges for plant synthetic biology.Quantitative biology (Beijing, China) · 2023Review
- Sources, dynamics in vivo, and application of astaxanthin and lutein in laying hens: A review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Review
- Metabolic engineering of astaxanthin-rich maize and its use in the production of biofortified eggs.Plant biotechnology journal · 2021Article
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of the high-value carotenoid astaxanthin, which is widely used in food and feed due to its strong antioxidant activity and colour, is less efficient in cereals than in model plants. Here, we report a new strategy for expressing β-carotene ketolase and hydroxylase genes from algae, yeasts and flowering plants in the whole seed using a seed-specific bidirectional promoter. Engineered maize events were backcrossed to inbred maize lines with yellow endosperm to generate progenies that accumulate astaxanthin from 47.76 to 111.82 mg/kg DW in seeds, and the maximum level is approximately sixfold higher than those in previous reports (16.2-16.8 mg/kg DW) in cereals. A feeding trial with laying hens indicated that they could take up astaxanthin from the maize and accumulate it in egg yolks (12.10-14.15 mg/kg) without affecting egg production and quality, as observed using astaxanthin from Haematococcus pluvialis. Storage stability evaluation analysis showed that the optimal conditions for long-term storage of astaxanthin-rich maize are at 4 °C in the dark. This study shows that co-expressing of functional genes driven by seed-specific bidirectional promoter could dramatically boost astaxanthin biosynthesis in every parts of kernel including embryo, aleurone layer and starch endosperm other than previous reports in the starch endosperm only. And the staple crop maize could serve as a cost-effective plant factory for reliably producing astaxanthin.
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