Evidence map›Paper›PMID 33780050›Full record

ArticleIndoor air2021

Investigation of bacterial and fungal communities in indoor and outdoor air of elementary school classrooms by 16S rRNA gene and ITS region sequencing.

Bong Gu Lee, Jun Il Yang, Euna Kim, Sun Woo Geum, Ju-Hyeong Park, Min-Kyeong Yeo

Open access · greenAbstract read
In one paragraph

Article in Indoor air, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 2 pooled it
2.4field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 2 syntheses or guidelines pooled it, 37 citations in OpenAlex.

  1. The Indoor Microbiome: Sampling, Analysis and Emerging Trends.Environmental microbiology reports · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Bong Gu LeeDepartment of Applied Environmental Science, Graduate School Kyung Hee University, Yongin-si, Korea.
Jun Il YangDepartment of Applied Environmental Science, Graduate School Kyung Hee University, Yongin-si, Korea.
Euna KimDepartment of Applied Environmental Science, Graduate School Kyung Hee University, Yongin-si, Korea.
Sun Woo GeumDepartment of Applied Environmental Science, Graduate School Kyung Hee University, Yongin-si, Korea.
Ju-Hyeong ParkRespiratory Health Division, National Institute for Occupational Safety and Health, Morgantown, WV, USA.ORCID 0000-0003-4419-9341
Min-Kyeong YeoDepartment of Applied Environmental Science, Graduate School Kyung Hee University, Yongin-si, Korea.ORCID 0000-0003-1353-7627
Kyung Hee University · KRNational Institute for Occupational Safety and Health · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

The advent of high-throughput sequencing methods allowed researchers to fully characterize microbial community in environmental samples, which is crucial to better understand their health effects upon exposures. In our study, we investigated bacterial and fungal community in indoor and outdoor air of nine classrooms in three elementary schools in Seoul, Korea. The extracted bacterial 16S rRNA gene and fungal ITS regions were sequenced, and their taxa were identified. Quantitative polymerase chain reaction for total bacteria DNA was also performed. The bacterial community was richer in outdoor air than classroom air, whereas fungal diversity was similar indoors and outdoors. Bacteria such as Enhydrobacter, Micrococcus, and Staphylococcus that are generally found in human skin, mucous membrane, and intestine were found in great abundance. For fungi, Cladosporium, Clitocybe, and Daedaleopsis were the most abundant genera in classroom air and mostly related to outdoor plants. Bacterial community composition in classroom air was similar among all classrooms but differed from that in outdoor air. However, indoor and outdoor fungal community compositions were similar for the same school but different among schools. Our study indicated the main source of airborne bacteria in classrooms was likely human occupants; however, classroom airborne fungi most likely originated from outdoors.

Indexed as

Air MicrobiologyEnvironmental MonitoringSchoolsAir Pollution, IndoorBacteriaCladosporiumDNA, BacterialFungiGenes, rRNAHousingHumansMicrobiotaMycobiomeRepublic of KoreaRNA, Ribosomal, 16SSeoulDNA, BacterialRNA, Ribosomal, 16Sclassroom airelementary school classroomsmicrobial communitiesnext-generation sequencing

Identifiers

PMID33780050
PMCPMC10230515
OpenAlexW3151676344

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.