ArticlePlant physiology2021
A seed coat-specific β-ketoacyl-CoA synthase, KCS12, is critical for preserving seed physical dormancy.
Article in Plant physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 37 citations in OpenAlex.
- Cracking the Hard Seed: Molecular Mechanisms and Multi-Omics Insights into Seed Dormancy and Germination in the GenusInternational journal of molecular sciences · 2026Review
- Seed fatty acid composition and physical dormancy in fire-prone ecosystems.Annals of botany · 2026Article
- Pleiotropic SNPs and genes linked to enhanced seed longevity and vigor for climate resilient pearl millet production.Frontiers in plant science · 2026Article
- Global Transcriptome Analysis Reveals the Molecular Mechanism Underlying Seed Physical Dormancy Formation inGenes · 2025Article
- ANTHOCYANIDIN REDUCTASE promotes physical dormancy in Medicago truncatula seeds.Plant physiology · 2025Article
- Genome-wide analysis of the KCS gene family in Medicago truncatula and their expression profile under various abiotic stress.Scientific reports · 2025Article
- A Comprehensive Analysis In Silico ofPlants (Basel, Switzerland) · 2024Article
- Association mapping unravels the genetic basis for drought related traits in different developmental stages of barley.Scientific reports · 2024Article
- Physical Seed Dormancy in Legumes: Molecular Advances and Perspectives.Plants (Basel, Switzerland) · 2024Review
- Identification of QTLs associated with very-long chain fatty acid (VLCFA) content via linkage mapping and BSA-seq in peanut.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Research advances on the hard seededness trait of soybean and the underlying regulatory mechanisms.Frontiers in plant science · 2024Review
- Physical seed dormancy in pea is genetically separable from seed coat thickness and roughness.Frontiers in plant science · 2024Article
- An ancestral role for 3-KETOACYL-COA SYNTHASE3 as a negative regulator of plant cuticular wax synthesis.The Plant cell · 2023Article
- Tackling functional redundancy of Arabidopsis fatty acid elongase complexes.Frontiers in plant science · 2023Article
- Genome-wide identification and expression analysis of theFrontiers in plant science · 2023Article
- Fine mapping and characterization of a major QTL for grain weight on wheat chromosome arm 5DL.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- CitWRKY28 and CitNAC029 promote the synthesis of cuticular wax by activating CitKCS gene expression in citrus fruit.Plant cell reports · 2022Article
- Effects of autotoxicity and allelopathy on seed germination and seedling growth inFrontiers in plant science · 2022Article
- Thirty Years of Mungbean Genome Research: Where Do We Stand and What Have We Learned?Frontiers in plant science · 2022Review
- Why so stubborn? MtKNOX4-regulated MtKCS12 manifests hardseededness.Plant physiology · 2021Article
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Authors and funding
14 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Physical dormancy in seeds exists widely in seed plants and plays a vital role in maintaining natural seed banks. The outermost cuticle of the seed coat forms a water-impermeable layer, which is critical for establishing seed physical dormancy. We previously set up the legume plant Medicago truncatula as an excellent model for studying seed physical dormancy, and our studies revealed that a class II KNOTTED-like homeobox, KNOX4, is a transcription factor critical for controlling hardseededness. Here we report the function of a seed coat β-ketoacyl-CoA synthase, KCS12. The expression level of KCS12 is significantly downregulated in the knox4 mutant. The KCS12 gene is predominantly expressed in the seed coat, and seed development in the M. truncatula kcs12 mutant is altered. Further investigation demonstrated that kcs12 mutant seeds lost physical dormancy and were able to absorb water without scarification treatment. Chemical analysis revealed that concentrations of C24:0 lipid polyester monomers are significantly decreased in mutant seeds, indicating that KCS12 is an enzyme that controls the production of very long chain lipid species in the seed coat. A chromatin immunoprecipitation assay demonstrated that the expression of KCS12 in the seed coat is directly regulated by the KNOX4 transcription factor. These findings define a molecular mechanism by which KNOX4 and KCS12 control formation of the seed coat and seed physical dormancy.
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