ArticleGenetics2021
A novel allele of the Arabidopsis thaliana MACPF protein CAD1 results in deregulated immune signaling.
Article in Genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- Unraveling the genetic basis of quantitative resistance to diseases in tomato: a meta-QTL analysis and mining of transcript profiles.Plant cell reports · 2024Pooled it
- Evaluation of the genes CYPEnvironmental science and pollution research international · 2026Article
- Plant-fungi interactions inProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Arabidopsis MACP2 contributes to autophagy induction by modulating starvation-induced reactive oxygen species homeostasis.Advanced biotechnology · 2025Article
- The Histidine-25-Arginine Mutation in the Rice MACPF Protein OsCAD1 Induces Cell Death and Activates Defense Responses in the Lesion Mimic Mutant spl17.Rice (New York, N.Y.) · 2025Article
- Catalytically inactive subgroup VIII receptor-like cytoplasmic kinases regulate the immune-triggered oxidative burst in Arabidopsis thaliana.Journal of experimental botany · 2025Article
- Functional divergence of CAD-like family genes in Saccharum complex under biotic and abiotic stress.Plant cell reports · 2025Article
- Subfamily C7 Raf-like kinases MRK1, RAF26, and RAF39 regulate immune homeostasis and stomatal opening in Arabidopsis thaliana.The New phytologist · 2024Article
- Article
- Genome-Wide Analysis of the Membrane Attack Complex and Perforin Genes and Their Expression Pattern under Stress in the Solanaceae.International journal of molecular sciences · 2023Article
- Overexpression of theInternational journal of molecular sciences · 2022Article
- Plant autoimmunity-fresh insights into an old phenomenon.Plant physiology · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
Abstract
Immune recognition in plants is governed by two major classes of receptors: pattern recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). Located at the cell surface, PRRs bind extracellular ligands originating from microbes (indicative of "non-self") or damaged plant cells (indicative of "infected-self"), and trigger signaling cascades to protect against infection. Located intracellularly, NLRs sense pathogen-induced physiological changes and trigger localized cell death and systemic resistance. Immune responses are under tight regulation in order to maintain homeostasis and promote plant health. In a forward-genetic screen to identify regulators of PRR-mediated immune signaling, we identified a novel allele of the membrane-attack complex and perforin (MACPF)-motif containing protein CONSTITUTIVE ACTIVE DEFENSE 1 (CAD1) resulting from a missense mutation in a conserved N-terminal cysteine. We show that cad1-5 mutants display deregulated immune signaling and symptoms of autoimmunity dependent on the lipase-like protein ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1), suggesting that CAD1 integrity is monitored by the plant immune system. We further demonstrate that CAD1 localizes to both the cytosol and plasma membrane using confocal microscopy and subcellular fractionation. Our results offer new insights into immune homeostasis and provide tools to further decipher the intriguing role of MACPF proteins in plants.
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Registered trials
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