RevieweLife2021
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.
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Review in eLife, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers.
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163citing papers in PubMed
–field-weighted citation impact
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Who cites it
163 citing papers in PubMed.
- An integrated open-source acquisition and analysis ecosystem for high-throughput single-molecule imaging.Nature methods · 2026Article
- Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET.Cell reports methods · 2026Article
- New paradigm of q-CAR drug development targeting disease-specific protein conformations.npj drug discovery · 2026Review
- ESMDynamic: Fast and accurate prediction of protein dynamic contact maps from single sequences.Nature communications · 2026Article
- ATP-driven conformational dynamics reveal hidden intermediates in a heterodimeric ABC transporter.eLife · 2026Article
- A Universal Protein Ladder for Standardization of Diverse FRET Assays.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Uninterrupted optical resolution of identical point scatterers undergoing nanometric changes in distance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- High-fidelity fast fluorescence lifetime imaging by event-based denoising.Nature biotechnology · 2026Article
- Dynamic positioning of Rpc34 winged helix in RNA polymerase III elongation complex for its stability with implications for reinitiation.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Structural heterogeneity of iron-sulfur cluster scaffold protein IscU: Metamorphic or partially folded?Structural dynamics (Melville, N.Y.) · 2026Review
- Dissecting mechanisms of ligand binding and conformational changes in the glutamine-binding protein.eLife · 2026Article
- Advances and challenges in multiscale biomolecular simulations: artificial intelligence-driven paradigm shift.Quantitative biology (Beijing, China) · 2026Article
- Toward a unifying mechanistic model of eukaryotic translation initiation through integrative single-molecule, structural, and computational insights.Current opinion in structural biology · 2026Review
- A combined PIE-FRET and FCS assay to monitor RNA dynamics and cleavage by SARS-CoV-2 Nsp15.Scientific reports · 2026Article
- Activation dynamics of a water-soluble human mu-opioid receptor.The Journal of biological chemistry · 2026Article
- BLeaching In-cell Single-molecule burstS (BLISS) reveals a small dynamic fraction of HP1α clusters in undifferentiated embryonic stem cells.bioRxiv : the preprint server for biology · 2026Article
- Single-Molecule Forster Resonance Energy Transfer With a Minimalistic 3D-Printed Setup and Dyes in the Blue-Green Spectral Region.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Toward a unified framework for determining conformational ensembles of disordered proteins.Nature methods · 2026Review
- Interfacial Interactions of Nanoparticles and Molecular Nanostructures with Model Membrane Systems: Mechanisms, Methods, and Applications.Membranes · 2026Review
- Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo.iScience · 2026Article
103 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
46 authors.
Eitan Lerner *Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, and The Center for Nanoscience and Nanotechnology, Faculty of Mathematics & Science, The Edmond J. Safra Campus, The Hebrew University of Jerusalem, Jerusalem, Israel.ORCID 0000-0002-3791-5277
Anders Barth *Lehrstuhl für Molekulare Physikalische Chemie, Heinrich-Heine-Universität, Düsseldorf, Germany.ORCID 0000-0003-3671-3072
Jelle Hendrix *Dynamic Bioimaging Lab, Advanced Optical Microscopy Centre and Biomedical Research Institute (BIOMED), Hasselt University, Diepenbeek, Belgium.ORCID 0000-0001-5731-1297
Benjamin AmbroseDepartment of Chemistry, University of Sheffield, Sheffield, United Kingdom.
Victoria BirkedalDepartment of Chemistry and iNANO center, Aarhus University, Aarhus, Denmark.
Scott C BlanchardDepartment of Structural Biology, St. Jude Children's Research Hospital, Memphis, United States.ORCID 0000-0003-2717-9365
Richard BörnerLaserinstitut HS Mittweida, University of Applied Science Mittweida, Mittweida, Germany.ORCID 0000-0001-8407-6624
Hoi Sung ChungLaboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.
Thorben CordesPhysical and Synthetic Biology, Faculty of Biology, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.ORCID 0000-0002-8598-5499
Timothy D CraggsDepartment of Chemistry, University of Sheffield, Sheffield, United Kingdom.ORCID 0000-0002-7121-0609
Ashok A DenizDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, United States.
Jiajie DiaoDepartment of Cancer Biology, University of Cincinnati School of Medicine, Cincinnati, United States.
Jingyi FeiDepartment of Biochemistry and Molecular Biology and The Institute for Biophysical Dynamics, University of Chicago, Chicago, United States.ORCID 0000-0002-9775-3820
Ruben L GonzalezDepartment of Chemistry, Columbia University, New York, United States.ORCID 0000-0002-1344-5581
Irina V GopichLaboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, United States.
Taekjip HaDepartment of Biophysics and Biophysical Chemistry, Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Howard Hughes Medical Institute, Baltimore, United States.ORCID 0000-0003-2195-6258
Christian A HankeLehrstuhl für Molekulare Physikalische Chemie, Heinrich-Heine-Universität, Düsseldorf, Germany.
Gilad HaranDepartment of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot, Israel.ORCID 0000-0003-1837-9779
Nikos S HatzakisDepartment of Chemistry & Nanoscience Centre, University of Copenhagen, Copenhagen, Denmark.
Sungchul HohngDepartment of Physics and Astronomy, and Institute of Applied Physics, Seoul National University, Seoul, Republic of Korea.
Seok-Cheol HongCenter for Molecular Spectroscopy and Dynamics, Institute for Basic Science and Department of Physics, Korea University, Seoul, Republic of Korea.
Thorsten HugelInstitute of Physical Chemistry and Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany.ORCID 0000-0003-3292-4569
Antonino IngargiolaDepartment of Chemistry and Biochemistry, and Department of Physiology, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0002-9348-1397
Chirlmin JooDepartment of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, Netherlands.
Achillefs N KapanidisBiological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom.
Harold D KimSchool of Physics, Georgia Institute of Technology, Atlanta, United States.
Ted LaurencePhysical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, United States.
Nam Ki LeeSchool of Chemistry, Seoul National University, Seoul, Republic of Korea.ORCID 0000-0002-6597-555X
Tae-Hee LeeDepartment of Chemistry, Pennsylvania State University, University Park, United States.
Edward A LemkeDepartments of Biology and Chemistry, Johannes Gutenberg University, Mainz, Germany.ORCID 0000-0002-0634-0503
Emmanuel MargeatCentre de Biologie Structurale (CBS), CNRS, INSERM, Universitié de Montpellier, Montpellier, France.
Jens MichaelisInstitüt of Biophysics, Ulm University, Ulm, Germany.
Xavier MichaletDepartment of Chemistry and Biochemistry, and Department of Physiology, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0001-6602-7693
Sua MyongDepartment of Biophysics, Johns Hopkins University, Baltimore, United States.
Daniel NettelsDepartment of Biochemistry and Department of Physics, University of Zurich, Zurich, Switzerland.
Thomas-Otavio PeulenDepartment of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, United States.
Evelyn PloetzPhysical Chemistry, Department of Chemistry, Center for Nanoscience (CeNS), Center for Integrated Protein Science Munich (CIPSM) and Nanosystems Initiative Munich (NIM), Ludwig-Maximilians-Universität, München, Germany.ORCID 0000-0003-0922-875X
Yair RazvagDepartment of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, and The Center for Nanoscience and Nanotechnology, Faculty of Mathematics & Science, The Edmond J. Safra Campus, The Hebrew University of Jerusalem, Jerusalem, Israel.
Nicole C RobbWarwick Medical School, University of Warwick, Coventry, United Kingdom.
Benjamin SchulerDepartment of Biochemistry and Department of Physics, University of Zurich, Zurich, Switzerland.ORCID 0000-0002-5970-4251
Hamid SoleimaninejadBiological Optical Microscopy Platform (BOMP), University of Melbourne, Parkville, Australia.
Chun TangCollege of Chemistry and Molecular Engineering, PKU-Tsinghua Center for Life Sciences, Beijing National Laboratory for Molecular Sciences, Peking University, Beijing, China.
Reza VafabakhshDepartment of Molecular Biosciences, Northwestern University, Evanston, United States.
Don C LambPhysical Chemistry, Department of Chemistry, Center for Nanoscience (CeNS), Center for Integrated Protein Science Munich (CIPSM) and Nanosystems Initiative Munich (NIM), Ludwig-Maximilians-Universität, München, Germany.ORCID 0000-0002-0232-1903
Claus Am SeidelLehrstuhl für Molekulare Physikalische Chemie, Heinrich-Heine-Universität, Düsseldorf, Germany.ORCID 0000-0002-5171-149X
Shimon WeissDepartment of Chemistry and Biochemistry, and Department of Physiology, University of California, Los Angeles, Los Angeles, United States.ORCID 0000-0002-0720-5426
Funding
Theoretical Studies On The Dynamic Aspects Of Macromolecular FunctionZIADK029019 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI SZABO, ATTILA · 2009 to 2024
$15.0MSingle molecule studies of protein binding and aggregationZIADK075113 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI CHUNG, HOI SUNG · 2015 to 2025
$10.7MImaging Protein Synthesis on the Ribosome using Single-Molecule FRETR01GM079238 · NIGMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI BLANCHARD, SCOTT C · 2006 to 2023
$7.0MThe Structural Dynamics of Translation InitiationR01GM084288 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI GONZALEZ, RUBEN L · 2009 to 2022
$4.4MNext-generation Fluorescent Probes for Biological ResearchR01GM098859 · NIGMS · WEILL MEDICAL COLL OF CORNELL UNIV · PI BLANCHARD, SCOTT C · 2012 to 2019
$2.9MInvestigation of energetics of sharp DNA bendingR01GM112882 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI KIM, HAROLD D · 2015 to 2023
$2.6MBiophysics of Protein Disorder and Complexity, Single Molecules to MesoscalesR35GM130375 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DENIZ, ASHOK A · 2019 to 2023
$2.4MQuantitative imaging of epitranscriptomic regulation mediated by RNA modificationDP2GM128185 · NIGMS · UNIVERSITY OF CHICAGO · PI FEI, JINGYI · 2017 to 2017
$2.4MStructural dynamics of RNAP-promoter complex in late transcription initiationR01GM130942 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WEISS, SHIMON · 2019 to 2022
$2.2MSingle-photon counting APD arrays for high-through-put single-molecule assaysR01GM095904 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GHIONI, MASSIMO ANTONIO, MICHALET, XAVIER · 2012 to 2016
$2.0MQuantitative analysis of metabotropic glutamate receptor activation and modulationR01GM140272 · NIGMS · NORTHWESTERN UNIVERSITY · PI VAFABAKHSH, REZA · 2020 to 2024
$1.7MThe structural dynamics of ribosomal frameshifting and ribosome rescueR01GM137608 · NIGMS · COLUMBIA UNIV NEW YORK MORNINGSIDE · PI GONZALEZ, RUBEN L · 2020 to 2023
$1.6MAgence Nationale de la Recherche ANR-17-CE09-0026-02Agence Nationale de la Recherche ANR-18-CE11-0004-02Agence Nationale de la Recherche ANR-19-CE44-0009-02Biotechnology and Biological Sciences Research Council BB/S008896/1Biotechnology and Biological Sciences Research Council BB/T008032/1Carlsbergfondet CF16-0797Deutsche Forschungsgemeinschaft CIBSS - EXC-2189 - project ID 390939984Deutsche Forschungsgemeinschaft GRK2062Deutsche Forschungsgemeinschaft PL696/4-1Deutsche Forschungsgemeinschaft SE 1195/21-1 (SPP 2191)Deutsche Forschungsgemeinschaft SFB1035Deutsche Forschungsgemeinschaft SFB 1129 240245660Deutsche Forschungsgemeinschaft SFB863Deutsche Forschungsgemeinschaft SPP2191 402723784European Research Council 638536European Research Council 671208European Research Council 819299European Research Council 860954European Research Council SMPFv2.0Human Frontier Science Program RGP0061/2019Independent Fund Denmark 6110-00623BIsrael Science Foundation 1250/19Israel Science Foundation 3565/20KU Leuven Special Research Fund C14/16/053National Key Research and Development Program of China 2018YFA0507700National Research Foundation of Korea 2019R1A2C2090896National Research Foundation of Korea NRF-2019R1A2C1089808National Research Foundation of Korea NRF-2019R1A2C2005209National Science Foundation 1818147National Science Foundation 1842951National Science Foundation 2004016NIGMS NIH HHS DP2 GM128185NIGMS NIH HHS R01 GM079238NIGMS NIH HHS R01 GM084288NIGMS NIH HHS R01 GM095904NIGMS NIH HHS R01 GM098859NIGMS NIH HHS R01 GM112882NIGMS NIH HHS R01 GM123164NIGMS NIH HHS R01 GM130793NIGMS NIH HHS R01 GM130942NIGMS NIH HHS R01 GM137608NIGMS NIH HHS R01 GM140272NIGMS NIH HHS R35 GM130375NIH HHS 128185NIH HHS GM079238NIH HHS GM084288NIH HHS GM095904NIH HHS GM130375NIH HHS GM130793NIH HHS GM130942NIH HHS GM137608Novo Nordisk NNF14CC0001Royal Society DKR00620Royal Society RGF\R1\180054Royal Society RGS\R2\180405UHasselt BOF fund R-10789Villum Fonden 18333Wellcome TrustWellcome Trust 110164/Z/15/Z
Abstract
Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-increasing number of groups has generated significant progress in sample preparation, measurement procedures, data analysis, algorithms and documentation. Several labs that employ smFRET approaches have joined forces to inform the smFRET community about streamlining how to perform experiments and analyze results for obtaining quantitative information on biomolecular structure and dynamics. The recent efforts include blind tests to assess the accuracy and the precision of smFRET experiments among different labs using various procedures. These multi-lab studies have led to the development of smFRET procedures and documentation, which are important when submitting entries into the archiving system for integrative structure models, PDB-Dev. This position paper describes the current 'state of the art' from different perspectives, points to unresolved methodological issues for quantitative structural studies, provides a set of 'soft recommendations' about which an emerging consensus exists, and lists openly available resources for newcomers and seasoned practitioners. To make further progress, we strongly encourage 'open science' practices.
Indexed as
Fluorescence Resonance Energy TransferMolecular BiologySingle Molecule Imagingbiochemistrybiomoleculeschemical biologycommunityconformationdynamicsFRETmolecular biophysicssingle-moleculestructural biology
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