ArticleFrontiers in veterinary science2020
An Integrated Molecular Approach to Untangling Host-Vector-Pathogen Interactions in Mosquitoes (Diptera: Culicidae) From Sylvan Communities in Mexico.
Article in Frontiers in veterinary science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Article
- DNA barcoding and blood meal profiling of Ethiopian mosquitoes (Diptera: Culicidae): insights into species identification and host preferences.Parasites & vectors · 2025Article
- An All-in-One Metabarcoding Approach to Mosquito and Arbovirus Xenosurveillance.Molecular ecology resources · 2025Article
- Spatial and Temporal Distribution of Mosquito Species (Culicidae) in a Ramsar Site, Fetzara Lake (Annaba, Algeria).Insects · 2025Article
- The Frontier of Entomo-Virology: Applications and Tools for Virus and Vector Surveillance.Pathogens (Basel, Switzerland) · 2025Review
- Seasonal haemosporidian detection in mosquitoes (Diptera: Culicidae) and their interactions with vertebrate hosts in a Mexican cloud forest.Parasitology research · 2024Article
- Article
- Article
- The first mitochondrial genome ofF1000Research · 2024Article
- Latest Advances in Arbovirus Diagnostics.Microorganisms · 2023Review
Corrections and comments
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Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There are ~240 species of Culicidae in Mexico, of which some are vectors of arthropod-borne viruses such as Zika virus, dengue virus, chikungunya virus, and West Nile virus. Thus, the identification of mosquito feeding preferences is paramount to understanding of vector-host-pathogen interactions that, in turn, can aid the control of disease outbreaks. Typically, DNA and RNA are extracted separately for animal (insects and blood meal hosts) and viral identification, but this study demonstrates that multiple organisms can be analyzed from a single RNA extract. For the first time, residual DNA present in standard RNA extracts was analyzed by DNA barcoding in concert with Sanger and next-generation sequencing (NGS) to identify both the mosquito species and the source of their meals in blood-fed females caught in seven sylvan communities in Chiapas State, Mexico. While mosquito molecular identification involved standard barcoding methods, the sensitivity of blood meal identification was maximized by employing short primers with NGS. In total, we collected 1,634 specimens belonging to 14 genera, 25 subgenera, and 61 morphospecies of mosquitoes. Of these, four species were new records for Mexico (
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Registered trials
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