ArticlePlant biotechnology journal2021
Spray-induced gene silencing for disease control is dependent on the efficiency of pathogen RNA uptake.
Article in Plant biotechnology journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 153 papers.
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Who cites it
153 citing papers in PubMed, 274 citations in OpenAlex.
- Ecology and evolutionary dynamics of the A-strain maize streak virus and the decade-long strategies targeting streak-resistant maize.Virus research · 2026Review
- Cross-kingdom RNA in plant-microbe interactions: from molecular interactions to next-generation crop protection.Stress biology · 2026Review
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- 3D mesoporous graphene nanoparticle-mediated transient silencing of susceptibility genes confers broad-spectrum disease resistance in crops.Plant communications · 2026Article
- Spray-Induced Gene Silencing (SIGS) of Dual-Target Genes (Journal of fungi (Basel, Switzerland) · 2026Article
- A conserved fungal Egh16-like effector suppresses host defense by disrupting ATP binding of wheat MPK3.Journal of integrative plant biology · 2026Article
- BcMFSR Is Required for Efficient Uptake of Exogenous dsRNA and Spray-Induced Gene Silencing in Botrytis cinerea.Molecular plant pathology · 2026Article
- Interactional Endocytosis and Transmembrane Transport Promote Cellular Internalization of Nano-Delivered RNA Drugs for Efficient Control of Crop Diseases.Plant biotechnology journal · 2026Article
- Nano-Enabled Precision Management of Plant Anthracnose: Mechanisms of Action, Application Advances, and Future Perspectives.Journal of fungi (Basel, Switzerland) · 2026Review
- Rational dsRNA design, scalable production and nanodelivery to enhance spray-induced gene silencing.Communications biology · 2026Review
- Nanoclay-Mediated RNA Interference Targeting Insecticide Receptors Enhances Whitefly Mortality and Reduces Fecundity.Molecular biotechnology · 2026Article
- Host-Induced Silencing of Rhizoctonia Solani 5-Enolpyruvylshikimate-3-Phosphate Synthase Impairs Its Virulence in Rice.Plant biotechnology journal · 2026Article
- LUCID: An Integrative Approach for Target Discovery and dsRNA Design in Plant Fungal Pathogens.Plant biotechnology journal · 2026Article
- Targeted Suppression of the Tomato PathogenJournal of fungi (Basel, Switzerland) · 2026Article
- Oomycete plant pathogens: biology, pathogenesis and emerging control strategies.Nature reviews. Microbiology · 2026Review
- Recent Advances in Pathogenicity and Biocontrol of PostharvestJournal of fungi (Basel, Switzerland) · 2026Review
- Cross-kingdom RNAi: a universal mechanism of inter-organismal communication with many unknowns.Journal of experimental botany · 2026Review
- Wheat miR408 and miR159 Weaken the Virulence ofPlants (Basel, Switzerland) · 2026Article
- Sspdhx Related to the Development and Virulence of Sclerotinia sclerotiorum Represents a Potential RNAi Target for Controlling Sclerotinia Disease.Molecular plant pathology · 2026Article
- Genomic and Effector-Based Insights Into Austropuccinia psidii-Host Interactions Informing RNAi and Resistance Development.Molecular plant pathology · 2026Review
93 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 4 countries.
Funding
Abstract
Recent discoveries show that fungi can take up environmental RNA, which can then silence fungal genes through environmental RNA interference. This discovery prompted the development of Spray-Induced Gene Silencing (SIGS) for plant disease management. In this study, we aimed to determine the efficacy of SIGS across a variety of eukaryotic microbes. We first examined the efficiency of RNA uptake in multiple pathogenic and non-pathogenic fungi, and an oomycete pathogen. We observed efficient double-stranded RNA (dsRNA) uptake in the fungal plant pathogens Botrytis cinerea, Sclerotinia sclerotiorum, Rhizoctonia solani, Aspergillus niger and Verticillium dahliae, but no uptake in Colletotrichum gloeosporioides, and weak uptake in a beneficial fungus, Trichoderma virens. For the oomycete plant pathogen, Phytophthora infestans, RNA uptake was limited and varied across different cell types and developmental stages. Topical application of dsRNA targeting virulence-related genes in pathogens with high RNA uptake efficiency significantly inhibited plant disease symptoms, whereas the application of dsRNA in pathogens with low RNA uptake efficiency did not suppress infection. Our results have revealed that dsRNA uptake efficiencies vary across eukaryotic microbe species and cell types. The success of SIGS for plant disease management can largely be determined by the pathogen's RNA uptake efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.