Evidence map›Paper›PMID 33774530›Full record

ArticleJournal of reproductive immunology2021

Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface.

Alison Chu, Su-Yin Kok, Jessica Tsui, Meng-Chin Lin, Brian Aguirre, Madhuri Wadehra

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of reproductive immunology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
  3. Characterization of microRNA Profiles inFrontiers in veterinary science · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Alison ChuDivision of Neonatology and Developmental Biology, Department of Pediatrics, David Geffen School of Medicine, University of California-Los Angeles, 10833 Le Conte Avenue, MDCC B2-411, Los Angeles, CA, 90095, USA. Electronic address: alisonchu@mednet.ucla.edu.
Su-Yin KokDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California-Los Angeles, 4525 MacDonald Research Laboratories, Los Angeles, CA, 90095, USA. Electronic address: skok@ucla.edu.
Jessica TsuiDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California-Los Angeles, 4525 MacDonald Research Laboratories, Los Angeles, CA, 90095, USA. Electronic address: jessicatsui92@gmail.com.
Meng-Chin LinDivision of Neonatology and Developmental Biology, Department of Pediatrics, David Geffen School of Medicine, University of California-Los Angeles, 10833 Le Conte Avenue, MDCC B2-411, Los Angeles, CA, 90095, USA. Electronic address: mlin@mednet.ucla.edu.
Brian AguirreDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California-Los Angeles, 4525 MacDonald Research Laboratories, Los Angeles, CA, 90095, USA. Electronic address: rayo95@ucla.edu.
Madhuri WadehraDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California-Los Angeles, 4525 MacDonald Research Laboratories, Los Angeles, CA, 90095, USA; Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California-Los Angeles, 4525 MacDonald Research Laboratories, Los Angeles, CA, 90095, USA. Electronic address: mwadehra@mednet.ucla.edu.
University of California, Los Angeles · US

Funding

Using EMP2 for Image Guided Drug Delivery for Breast CancerR01CA163971 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI OLAFSEN, TOVE, WADEHRA, MADHURI · 2012 to 2016
$1.6M
The role of IL10 in early vascularity, endothelial dysfunction, and programming of adult cardiovascular diseaseK08HD093874 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHU, ALISON · 2018 to 2020
$536k
NCI NIH HHS R01 CA163971NICHD NIH HHS K08 HD093874
6 · The paper itself

Abstract

Epithelial membrane protein 2 (EMP2) is a tetraspan membrane protein that has been revealed in cancer and placental models to mediate a number of vascular responses. Recently, Emp2 modulation has been shown to have an immunologic effect on uterine NK cell recruitment in the mouse placenta. Given the importance of immune cell populations on both placental vascularization and maternal immune tolerance of the developing fetus, we wanted to better characterize the immunologic effects of Emp2 at the placental-fetal interface. We performed flow cytometry of WT and Emp2 KO C57Bl/6 mouse uterine horns at GD12.5 to characterize immune cell populations localized to the various components of the maternal-fetal interface. We found that Emp2 KO decidua and placenta showed an elevated overall percentage of CD45+ cells compared to WT. Characterization of CD45+ cells in the decidua of Emp2 KO dams revealed an increase in NK cells, whereas in the placenta, Emp2 KO dams showed an increased percentage of M1 macrophages (with an increased ratio of M1/M2 macrophages). Given the differences detected in uNK cell populations in the decidua, we further characterized the interaction between Emp2 genetic KO and NK cell deletion via anti-asialo GM1 antibody injections. While the double knock-out of Emp2 and NK cells did not alter individual pup birthweight, it significantly reduced total litter weight and size by ∼50 %. In conclusion, Emp2 appears to regulate uNK and macrophage cell populations in pregnancy.

Indexed as

Histocompatibility, Maternal-FetalAnimalsDeciduaFemaleImmune ToleranceImmunity, InnateKiller Cells, NaturalMacrophagesMembrane GlycoproteinsMiceMice, KnockoutModels, AnimalPregnancyEmp2 protein, mouseMembrane GlycoproteinsEpithelial membrane protein 2MacrophagesPlacentaUterine natural killer cells

Identifiers

PMID33774530
PMCPMC8722772
OpenAlexW3133689471

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.