Evidence map›Paper›PMID 33774011›Full record

ArticleDevelopmental biology2021

Early cis-regulatory events in the formation of retinal horizontal cells.

Estie Schick, Kevin C Gonzalez, Pooja Dutta, Kazi Hossain, Miruna G Ghinia Tegla, Mark M Emerson

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Estie SchickBiology PhD Program, Graduate Center, City University of New York, New York, NY, 10016, USA.
Kevin C GonzalezDepartment of Biology, The City College of New York, City University of New York, New York, NY, 10031, USA.
Pooja DuttaDepartment of Biology, The City College of New York, City University of New York, New York, NY, 10031, USA.
Kazi HossainDepartment of Biology, The City College of New York, City University of New York, New York, NY, 10031, USA.
Miruna G Ghinia TeglaDepartment of Biology, The City College of New York, City University of New York, New York, NY, 10031, USA.
Mark M EmersonBiology PhD Program, Graduate Center, City University of New York, New York, NY, 10016, USA; Department of Biology, The City College of New York, City University of New York, New York, NY, 10031, USA; Biochemistry PhD Program, Graduate Center, City University of New York, New York, NY, 10016, USA. Electronic address: memerson@ccny.cuny.edu.
City College of New York · USThe Graduate Center, CUNY · US

Funding

Cellular/Molecular Basis of Development: Research CenterG12MD007603 · NIMHD · CITY COLLEGE OF NEW YORK · PI PEZZANO, MARK · 2012 to 2017
$11.5M
MARC HONORS UNDERGRADUATE RESEARCH TRAINING PROGRAMT34GM007639 · NIGMS · CITY COLLEGE OF NEW YORK · PI LEVITT, JONATHAN B · 1985 to 2022
$4.7M
Transcriptional Regulation of Cone Photoreceptor GenesisR01EY024982 · NEI · CITY COLLEGE OF NEW YORK · PI EMERSON, MARK M · 2015 to 2023
$3.2M
NEI NIH HHS R01 EY024982NIGMS NIH HHS T34 GM007639NIMHD NIH HHS G12 MD007603
6 · The paper itself

Abstract

During retinal development, multipotent and restricted progenitor cells generate all of the neuronal cells of the retina. Among these are horizontal cells, which are interneurons that modulate the light-induced signal from photoreceptors. This study utilizes the identification of novel cis-regulatory elements as a method to examine the gene regulatory networks that direct the development of horizontal cells. Here we describe a screen for cis-regulatory elements, or enhancers, for the horizontal cell-associated genes PTF1A, ONECUT1 (OC1), TFAP2A (AP2A), and LHX1. The OC1ECR22 and Tfap2aACR5 elements were shown to be potential enhancers for OC1 and TFAP2A, respectively, and to be specifically active in developing horizontal cells. The OC1ECR22 element is activated by PTF1A and RBPJ, which translates to regulation of OC1 expression and suggests that PTF1A is a direct activator of OC1 expression in developing horizontal cells. The region within the Tfap2aACR5 element that is responsible for its activation was determined to be a 100 bp sequence named Motif 4. Both OC1ECR22 and Tfap2aACR5 are negatively regulated by the nuclear receptors THRB and RXRG, as is the expression of OC1 and AP2A, suggesting that nuclear receptors may have a role in the negative regulation of horizontal cell development.

Indexed as

AnimalsCell DifferentiationChick EmbryoGene ExpressionGene Expression Regulation, DevelopmentalGene Regulatory NetworksHomeodomain ProteinsLIM-Homeodomain ProteinsNeuronsOnecut Transcription FactorsRetinaRetinal Horizontal CellsStem CellsTranscription Factor AP-2Transcription FactorsHomeodomain ProteinsLIM-Homeodomain ProteinsOnecut Transcription FactorsTranscription Factor AP-2transcription factor PTF1Transcription FactorsChickCis-regulationElectroporationEnhancer elementHorizontal cellRetina

Identifiers

PMID33774011
PMCPMC8522209
OpenAlexW3138307102

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.