Evidence map›Paper›PMID 33773561›Full record

ArticleAsian Pacific journal of cancer prevention : APJCP2021

Investigation the Role of Autophagy in Non-Small Cell Lung Cancer.

Minoo Pargol, Shima Zare Karizi, Masoumeh Akbari, Bahareh Nourmohammadi, Mohammad Behgam Shadmehr, Morteza Karimipoor, Shohreh Zare Karizi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Asian Pacific journal of cancer prevention : APJCP, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Minoo PargolDepartment of Genetics and Biotechnology, School of Biological Science, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.ORCID 0000-0002-7920-2347
Shima Zare KariziDepartment of Genetics and Biotechnology, School of Biological Science, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.
Masoumeh AkbariDepartment of Genetics and Biotechnology, School of Biological Science, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.
Bahareh NourmohammadiMolecular Medicine Department, Biotechnology Research center, Pasteur Institute of Iran, Tehran, Iran.ORCID 0000-0002-6013-6802
Mohammad Behgam ShadmehrTracheal Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Morteza KarimipoorMolecular Medicine Department, Biotechnology Research center, Pasteur Institute of Iran, Tehran, Iran.ORCID 0000-0002-2406-5963
Shohreh Zare KariziDepartment of Genetics and Biotechnology, School of Biological Science, Varamin Pishva Branch, Islamic Azad University, Varamin, Iran.ORCID 0000-0002-0305-8588
Pasteur Institute of Iran · IRMasih Daneshvari Hospital · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveRecent studies have shown the role of autophagy in different types of cancer including lung cancer. MicroRNAs are considered as key factors in regulation of autophagy related genes. miR-30d, miR-204-5p and miR-20a are regulatory markers which can suppress the expression of beclin1, LC3, bcl2 and ULK1 as their target genes and they lead to decrement of autophagy in human cancer cells. Moreover, epigenetic modifications DNA methylation has been indicated in regulation of autophagy in different stages of cancer.

methodsIn this study, the expression levels of miR-30d, miR-204-5p and miR-20a as well as their target genes were analyzed in 30 non-small cell lung cancers (NSCLCs) patients sample and adjacent normal tissues by real-time qPCR. In addition, DNA methylation of beclin1, LC3, bcl2 and ULK1 genes were assessed by MS-HRM method.

resultsMiR-30d (p value= 0.01) and miR-204-5p (P=0.048) significantly down-regulated in tumor samples compared to normal adjacent tissues, while there was no significant change in expression level of miR-20a. On the other hand, target genes expression level was significantly increased in NSCLC tissues, however methylation pattern of the target gene promoters, did not show any significant alteration.

conclusionThese results indicate roles for miR-30d, miR-204-5p as tumor suppressor genes as well as target genes as oncogenes in NSCLC patients. Although these factors may have a significant role in NSCLC progression, further studies are necessary to investigate the implications of these findings for treatment of lung cancer. <br />.

Indexed as

Adenocarcinoma of LungAutophagyAutophagy-Related Protein-1 HomologBeclin-1Carcinoma, Non-Small-Cell LungCarcinoma, Squamous CellDNA MethylationFemaleHumansIntracellular Signaling Peptides and ProteinsLung NeoplasmsMaleMicroRNAsMicrotubule-Associated ProteinsMiddle AgedProto-Oncogene Proteins c-bcl-2Autophagy-Related Protein-1 HomologBCL2 protein, humanBeclin-1BECN1 protein, humanIntracellular Signaling Peptides and ProteinsMAP1LC3A protein, humanMicroRNAsMicrotubule-Associated ProteinsMIRN204 microRNA, humanMIRN20a microRNA, humanMIRN30d microRNA, humanProto-Oncogene Proteins c-bcl-2ULK1 protein, humanAutophagyDNA MethylationMicroRNAsnon-small cell lung cancer

Identifiers

PMID33773561
PMCPMC8286697
OpenAlexW3147492267

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.