ArticleStem cell research2021
Matrigel is required for efficient differentiation of isolated, stem cell-derived otic vesicles into inner ear organoids.
Article in Stem cell research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Inner Ear Organoids: Recent Progress and Challenges.Stem cell reviews and reports · 2026Review
- Redefining 3D cell culture: Human methacryloyl platelet lysates hydrogels for reliable, consistent, and ethical applications.Materials today. Bio · 2026Article
- Bioprinted Bladder Cancer Organoids Model System for Prediction of Chemotherapy Response and Drug Screening.International journal of molecular sciences · 2026Article
- Organoid research: new concepts and new technologies.Burns & trauma · 2026Review
- Advances and prospects of 3D air-liquid interface cultured middle ear epithelial cells and inner ear organoids in otologic diseases.Frontiers in bioengineering and biotechnology · 2026Review
- 3D endothelial network formation in hydrogels improved by stromal cells and specific growth factors.Scientific reports · 2025Article
- Study Models for Non-Syndromic Hearing Loss.Cells · 2025Review
- Retinoic acid signaling guides the efficiency of inner ear organoid-genesis and governs sensory-nonsensory fate specification.Stem cell reports · 2025Article
- Inner Ear Organoid as a Preclinical Model of Hearing Regeneration: Progress and Applications.Stem cell reviews and reports · 2025Review
- The Reconstruction of Peripheral Auditory Circuit: Recent Advances and Future Challenges.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Review
- Exploiting Matrix Stiffness to Overcome Drug Resistance.ACS biomaterials science & engineering · 2024Review
- Mapping the developmental potential of mouse inner ear organoids at single-cell resolution.iScience · 2024Article
- Modeling, applications and challenges of inner ear organoid.Smart medicine · 2024Review
- Morphogenesis and development of human telencephalic organoids in the absence and presence of exogenous extracellular matrix.The EMBO journal · 2023Article
- Review
- Emerging organoid-immune co-culture models for cancer research: from oncoimmunology to personalized immunotherapies.Journal for immunotherapy of cancer · 2023Review
- Growth and differentiation of human induced pluripotent stem cell (hiPSC)-derived kidney organoids using fully synthetic peptide hydrogels.Bioactive materials · 2023Article
- Current advances in biomaterials for inner ear cell regeneration.Frontiers in neuroscience · 2023Review
- Modelling inner ear development and disease using pluripotent stem cells - a pathway to new therapeutic strategies.Disease models & mechanisms · 2022Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Inner ear organoids derived from pluripotent stem cells could be a useful model system to study development, disease, and regeneration. However, there is considerable heterogeneity in the size, morphology, and efficiency of organoid production using standard protocols. Greater control of the culture microenvironment could decrease heterogeneity and increase the yield of organoids. Animal-derived otic vesicles show some autonomy during development and can differentiate into cochlear and vestibular domains in mesenchyme-free ex vivo culture. Therefore, we investigated whether stem cell-derived otic vesicles can autonomously generate inner ear organoids. Isolated, stem cell-derived vesicles grew into cyst-like organoids with high efficiency, over 90%, when embedded in droplets of the basement membrane matrix Matrigel. Though nearly all vesicles within the aggregate were competent to mature into organoids, the efficiency of organoid production depended on the stage of vesicle isolation and required supplementation with Matrigel.
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Registered trials
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