Evidence map›Paper›PMID 33772629›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2021

Complement regulation in tenocytes under the influence of leukocytes in an indirect co-culture model.

Sandeep Silawal, Benjamin Kohl, Georg Girke, Tobias Schneider, Gundula Schulze-Tanzil

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In one paragraph

Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Animal model for tendinopathy.Journal of orthopaedic translation · 2023
    Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Sandeep SilawalInstitute of Anatomy and Cell Biology, Paracelsus Private Medical University, Nuremberg and Salzburg, General Hospital Nuremberg, Prof. Ernst Nathan Str. 1, 90419, Nuremberg, Germany.
Benjamin KohlDepartment of Traumatology and Reconstructive Surgery, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Zu Berlin and Berlin Institute of Health, Campus Benjamin Franklin, Hindenburgdamm 30, 12203, Berlin, Germany.
Georg GirkeDepartment of Traumatology and Reconstructive Surgery, Charité - Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Zu Berlin and Berlin Institute of Health, Campus Benjamin Franklin, Hindenburgdamm 30, 12203, Berlin, Germany.
Tobias SchneiderInstitute of Anatomy and Cell Biology, Paracelsus Private Medical University, Nuremberg and Salzburg, General Hospital Nuremberg, Prof. Ernst Nathan Str. 1, 90419, Nuremberg, Germany.
Gundula Schulze-TanzilInstitute of Anatomy and Cell Biology, Paracelsus Private Medical University, Nuremberg and Salzburg, General Hospital Nuremberg, Prof. Ernst Nathan Str. 1, 90419, Nuremberg, Germany. gundula.schulze@pmu.ac.at.
Nuremberg Hospital · DECharité - Universitätsmedizin Berlin · DEFreie Universität Berlin · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThe present in vitro study was undertaken to learn about the effects of leukocytes on tenocytes in respect to complement regulation simulating an inflammatory scenario of the traumatized tissue.

methodsHuman hamstring tendon-derived tenocyte monolayers were co-cultured indirectly with human leukocytes (either Peripheral Blood Mononuclear Cells [PBMCs] or neutrophils) using a transwell system with/without (+ /

resultsC5aR gene expression was significantly induced by TNFα after 4 h, but impaired in the presence of leukocytes + TNFα after 24 h. At 4 h, PBMCs activated by TNFα induced the CRP CD46 gene expression. However, CD55 was significantly suppressed after 24 h by neutrophils + /

conclusionTNFα might represent a crucial soluble mediator exerting diverse time-dependent effects on tenocyte complement regulation.

Indexed as

AdultAntigens, CDCells, CulturedCoculture TechniquesComplement System ProteinsFemaleHumansLeukocytes, MononuclearMaleMiddle AgedReceptor, Anaphylatoxin C5aTenocytesTumor Necrosis Factor-alphaAntigens, CDC5AR1 protein, humanComplement System ProteinsReceptor, Anaphylatoxin C5aTumor Necrosis Factor-alphaComplement regulationComplement systemIndirect co-cultureLeukocytesTendinopathyTenocytesTNFα

Identifiers

PMID33772629
OpenAlexW3150167363

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.