Evidence map›Paper›PMID 33772460›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Monitoring Axonal Degeneration in Human Pluripotent Stem Cell Models of Hereditary Spastic Paraplegias.

Xue-Jun Li, Yongchao Mou, Conrad Milton, Zhenyu Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Xue-Jun LiDepartment of Biomedical Sciences, University of Illinois College of Medicine Rockford, Rockford, IL, USA. xjli23@uic.edu.
Yongchao MouDepartment of Biomedical Sciences, University of Illinois College of Medicine Rockford, Rockford, IL, USA.
Conrad MiltonDepartment of Biomedical Sciences, University of Illinois College of Medicine Rockford, Rockford, IL, USA.
Zhenyu ChenDepartment of Biomedical Sciences, University of Illinois College of Medicine Rockford, Rockford, IL, USA.
Illinois College · USUniversity of Illinois Chicago · US

Funding

Uncover the role of glia-neuron crosstalk in hereditary spastic paraplegiasR01NS118066 · NINDS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI LI, XUE-JUN · 2020 to 2024
$1.9M
Rescuing axonal defects of human cortical neurons in hereditary spastic paraplegiasR21NS109837 · NINDS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI LI, XUE-JUN · 2019 to 2019
$440k
Role of atlastin-1 in axonal development and degeneration of human neuronsR21NS089042 · NINDS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI LI, XUE-JUN · 2014 to 2015
$438k
NINDS NIH HHS R01 NS118066NINDS NIH HHS R21 NS089042NINDS NIH HHS R21 NS109837
6 · The paper itself

Abstract

Axonal degeneration underlies many debilitating diseases including hereditary spastic paraplegias (HSPs). HSPs are a large heterogeneous group of neurodegenerative diseases characterized by axonopathy involving the long corticospinal tract. How axons of these cortical projection neurons specifically degenerate in HSPs remains largely unclear partially due to the lack of human models to monitor the dynamic process of axonal degeneration. With the development of induced pluripotent stem cell (iPSC) technology, patient-specific iPSCs are successfully generated from HSP patients, providing a unique paradigm to study the axonal degeneration in patient-derived neurons in live cultures. In this chapter, we will summarize the procedures to examine axonal defects in iPSC models of HSPs and discuss the challenges and future applications in order to rescue axonal degeneration in HSPs.

Indexed as

Induced Pluripotent Stem CellsSpastic Paraplegia, HereditaryAxonsHumansNeuronsAxonal degenerationAxon transportCortical projection neuronsHereditary spastic paraplegiaHuman stem cellsTransport

Identifiers

PMID33772460
PMCPMC8473581
OpenAlexW3139066410

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.