ArticlePLoS pathogens2021
Cytotoxicity of Vibrio parahaemolyticus AHPND toxin on shrimp hemocytes, a newly identified target tissue, involves binding of toxin to aminopeptidase N1 receptor.
Article in PLoS pathogens, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 48 citations in OpenAlex.
- Network Clustering Approach Reveals Key Proteins and Biological Functions in the Response of Whiteleg Shrimp (Penaeus vannamei) to Acute Hepatopancreatic Necrosis Disease.Marine biotechnology (New York, N.Y.) · 2026Article
- Single-cell transcriptomics of Pacific white shrimp (Frontiers in immunology · 2026Article
- Report on Vibrio Species Contamination in Shrimp From the Coast of Pangandaran, West Java, Indonesia.Environmental microbiology reports · 2025Article
- Investigating a novel β-glucan source to enhance disease resistance in Pacific white shrimp (Penaeus vannamei).Scientific reports · 2025Article
- Transcriptomic insights into the resistance mechanism of Penaeus vannamei against highly lethal Vibrio parahaemolyticus.Scientific reports · 2025Article
- Viral circular RNA-encoded protein, ceVP28, divulges an antiviral response in invertebrates.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The effects ofFish and shellfish immunology reports · 2023Article
- A bacterial binary toxin system that kills both insects and aquatic crustaceans: Photorhabdus insect-related toxins A and B.PLoS pathogens · 2023Review
- Kruppel homolog 1 modulates ROS production and antimicrobial peptides expression in shrimp hemocytes during infection by theFrontiers in immunology · 2023Article
- Comparative transcriptomic analysis of gill reveals genes belonging to mTORC1 signaling pathway associated with the resistance trait of shrimp to VPFrontiers in immunology · 2023Article
- The Hippo-Yki Signaling Pathway Positively Regulates Immune Response againstInternational journal of molecular sciences · 2022Article
- Haloalkaline Lipase from Bacillus flexus PU2 Efficiently Inhibits Biofilm Formation of Aquatic Pathogen Vibrio parahaemolyticus.Probiotics and antimicrobial proteins · 2022Article
- Review
- Expression of the AHPND Toxins PirAInternational journal of molecular sciences · 2022Article
- Functional Analysis of Hypothetical Proteins ofBioinformatics and biology insights · 2022Article
- Identification and Expression Analysis of an Interacting Protein (LvFABP) that MediatesFrontiers in immunology · 2022Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acute hepatopancreatic necrosis disease (AHPND) caused by PirABVP-producing strain of Vibrio parahaemolyticus, VPAHPND, has seriously impacted the shrimp production. Although the VPAHPND toxin is known as the VPAHPND virulence factor, a receptor that mediates its action has not been identified. An in-house transcriptome of Litopenaeus vannamei hemocytes allows us to identify two proteins from the aminopeptidase N family, LvAPN1 and LvAPN2, the proteins of which in insect are known to be receptors for Cry toxin. The membrane-bound APN, LvAPN1, was characterized to determine if it was a VPAHPND toxin receptor. The increased expression of LvAPN1 was found in hemocytes, stomach, and hepatopancreas after the shrimp were challenged with either VPAHPND or the partially purified VPAHPND toxin. LvAPN1 knockdown reduced the mortality, histopathological signs of AHPND in the hepatopancreas, and the number of virulent VPAHPND bacteria in the stomach after VPAHPND toxin challenge. In addition, LvAPN1 silencing prevented the toxin from causing severe damage to the hemocytes and sustained both the total hemocyte count (THC) and the percentage of living hemocytes. We found that the rLvAPN1 directly bound to both rPirAVP and rPirBVP toxins, supporting the notion that silencing of LvAPN1 prevented the VPAHPND toxin from passing through the cell membrane of hemocytes. We concluded that the LvAPN1 was involved in AHPND pathogenesis and acted as a VPAHPND toxin receptor mediating the toxin penetration into hemocytes. Besides, this was the first report on the toxic effect of VPAHPND toxin on hemocytes other than the known target tissues, hepatopancreas and stomach.
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