ReviewMolecular systems biology2021
A guidebook for DISCO tissue clearing.
Review in Molecular systems biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed.
- A uniform tissue-clearing framework and mesoSPIM-ultra enable cm-scale single-neuron tracing.bioRxiv : the preprint server for biology · 2026Article
- Article
- Article
- SynClear: A one-step synchronous clearing and labeling strategy for multiscale 3D brain mapping.Materials today. Bio · 2026Article
- Advanced tissue clearing and three-dimensional imaging approaches to visualize neural innervation in the rat knee joints.Npj imaging · 2026Article
- A Novel Rapid 3D Tissue-Clearing and Staining Approach for Enteric Neurovascular Imaging and Pathology Applications.Diagnostics (Basel, Switzerland) · 2026Article
- Advances in Optical Tissue Clearing for Three-Dimensional Ocular Imaging and Analysis.Journal of ophthalmology · 2026Review
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- A Simple and Fast Optical Clearing Method for Whole-Mount Fluorescence In Situ Hybridization (FISH) Imaging.Journal of biophotonics · 2025Article
- A novel protein-preserving passive tissue clearing approach using sodium cholate and urea for whole-organ imaging.Experimental & molecular medicine · 2025Article
- ADAPT-3D:accelerated deep adaptable processing of tissue for 3-dimensional fluorescence tissue imaging for research and clinical settings.Scientific reports · 2025Article
- Multiplexed, image-based pooled screens in primary cells and tissues with PerturbView.Nature biotechnology · 2025Article
- Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures.STAR protocols · 2024Article
- Whole-Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging.Chemical & biomedical imaging · 2024Article
- Novel strategy to preserve valve morphology for 3-dimensional analysis of rat pulmonary valves.JTCVS structural and endovascular · 2024Article
- Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy.Scientific reports · 2024Article
- Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders.Molecular psychiatry · 2024Review
- Virtual reality-empowered deep-learning analysis of brain cells.Nature methods · 2024Article
- Simplifying deep learning to enhance accessibility of large-scale 3D brain imaging analysis.Nature methods · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histological analysis of biological tissues by mechanical sectioning is significantly time-consuming and error-prone due to loss of important information during sample slicing. In the recent years, the development of tissue clearing methods overcame several of these limitations and allowed exploring intact biological specimens by rendering tissues transparent and subsequently imaging them by laser scanning fluorescence microscopy. In this review, we provide a guide for scientists who would like to perform a clearing protocol from scratch without any prior knowledge, with an emphasis on DISCO clearing protocols, which have been widely used not only due to their robustness, but also owing to their relatively straightforward application. We discuss diverse tissue-clearing options and propose solutions for several possible pitfalls. Moreover, after surveying more than 30 researchers that employ tissue clearing techniques in their laboratories, we compiled the most frequently encountered issues and propose solutions. Overall, this review offers an informative and detailed guide through the growing literature of tissue clearing and can help with finding the easiest way for hands-on implementation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.