ArticlePhytopathology research2020
Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones.
Article in Phytopathology research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.Nature communications · 2026Article
- Engineering the 3'-UTR of Tobacco Vein Mottling Virus to Confer Cross-Protection Against Potyviruses.Molecular plant pathology · 2026Article
- Defensin-like Protein Homologues Participate in Antiviral Resistance inLife (Basel, Switzerland) · 2026Article
- Resveratrol synthase homologs participate in infection ofFrontiers in microbiology · 2025Article
- Cu/Zn superoxide dismutase homologs participate inFrontiers in microbiology · 2025Article
- N6-adenosine-methyltransferase MTA70-like participates antiviral responses inFrontiers in microbiology · 2025Article
- Use of Bacterial Toxin-Antitoxin Systems as Biotechnological Tools in Plants.International journal of molecular sciences · 2024Article
- 3'UTR of tobacco vein mottling virus regulates downstream GFP expression and changes in host gene expression.Frontiers in microbiology · 2024Article
- Assembly of plant virus agroinfectious clones using biological material or DNA synthesis.STAR protocols · 2022Article
- Proteome expansion in the Potyviridae evolutionary radiation.FEMS microbiology reviews · 2022Review
- The PilB-PilZ-FimX regulatory complex of the Type IV pilus from Xanthomonas citri.PLoS pathogens · 2021Article
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Authors and funding
7 authors.
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Abstract
An unprecedented number of viruses have been discovered by leveraging advances in high-throughput sequencing. Infectious clone technology is a universal approach that facilitates the study of biology and role in disease of viruses. In recent years homology-based cloning methods such as Gibson assembly have been used to generate virus infectious clones. We detail herein the preparation of home-made cloning materials for Gibson assembly. The home-made materials were used in one-step generation of the infectious cDNA clone of a plant RNA virus into a T-DNA binary vector. The clone was verified by a single Illumina reaction and a de novo read assembly approach that required no primer walking, custom primers or reference sequences. Clone infectivity was finally confirmed by
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