ArticleThe Plant journal : for cell and molecular biology2021
The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases.
Article in The Plant journal : for cell and molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 32 citations in OpenAlex.
- Auxin Biosynthesis, Transport, Signaling, and Its Roles in Plant Leaf Morphogenesis.Plants (Basel, Switzerland) · 2025Review
- Design and construction of a low-cost, low-input Open Top Chamber field warming setup to assess aboveground plant response to global warming.Frontiers in plant science · 2025Article
- Transcriptomic profiling reveals histone acetylation-regulated genes involved in somatic embryogenesis in Arabidopsis thaliana.BMC genomics · 2024Article
- Using a thermal gradient table to study plant temperature signalling and response across a temperature spectrum.Plant methods · 2024Article
- A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Organ-specific transcriptional regulation by HFR1 and HY5 in response to shade in Arabidopsis.Frontiers in plant science · 2024Article
- High temperature restricts cell division and leaf size by coordination of PIF4 and TCP4 transcription factors.Plant physiology · 2022Article
- Nitrilases NIT1/2/3 Positively Regulate Flowering by InhibitingFrontiers in plant science · 2022Article
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19 authors at 7 institutions in 5 countries.
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Abstract
Temperature passively affects biological processes involved in plant growth. Therefore, it is challenging to study the dedicated temperature signalling pathways that orchestrate thermomorphogenesis, a suite of elongation growth-based adaptations that enhance leaf-cooling capacity. We screened a chemical library for compounds that restored hypocotyl elongation in the pif4-2-deficient mutant background at warm temperature conditions in Arabidopsis thaliana to identify modulators of thermomorphogenesis. The small aromatic compound 'Heatin', containing 1-iminomethyl-2-naphthol as a pharmacophore, was selected as an enhancer of elongation growth. We show that ARABIDOPSIS ALDEHYDE OXIDASES redundantly contribute to Heatin-mediated hypocotyl elongation. Following a chemical proteomics approach, the members of the NITRILASE1-subfamily of auxin biosynthesis enzymes were identified among the molecular targets of Heatin. Our data reveal that nitrilases are involved in promotion of hypocotyl elongation in response to high temperature and Heatin-mediated hypocotyl elongation requires the NITRILASE1-subfamily members, NIT1 and NIT2. Heatin inhibits NIT1-subfamily enzymatic activity in vitro and the application of Heatin accordingly results in the accumulation of NIT1-subfamily substrate indole-3-acetonitrile in vivo. However, levels of the NIT1-subfamily product, bioactive auxin (indole-3-acetic acid), were also significantly increased. It is likely that the stimulation of hypocotyl elongation by Heatin might be independent of its observed interaction with NITRILASE1-subfamily members. However, nitrilases may contribute to the Heatin response by stimulating indole-3-acetic acid biosynthesis in an indirect way. Heatin and its functional analogues present novel chemical entities for studying auxin biology.
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