Evidence map›Paper›PMID 33768511›Full record

ArticleHuman cell2021

Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes and PEDF secretion.

Jinhui Zhang, Wenya Fan, Lingling Neng, Bei Chen, Bin Zuo, Wei Lu

Abstract read
PubMed Publisher
In one paragraph

Article in Human cell, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jinhui ZhangDepartment of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No 1 Jianshe Road, Zhengzhou, 450052, China. zhangjinhui662008@126.com.
Wenya FanDepartment of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No 1 Jianshe Road, Zhengzhou, 450052, China.
Lingling NengDepartment of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No 1 Jianshe Road, Zhengzhou, 450052, China.
Bei ChenDepartment of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No 1 Jianshe Road, Zhengzhou, 450052, China.
Bin ZuoDepartment of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No 1 Jianshe Road, Zhengzhou, 450052, China.
Wei LuDepartment of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital of Zhengzhou University, No 1 Jianshe Road, Zhengzhou, 450052, China. luweimd@hotmail.com.
First Affiliated Hospital of Zhengzhou University · CN

Funding

Funds from Scientific Studies of Higher Education Institution in Henan 18A3210015National Natural Science Youth Foundation of China 81700905
6 · The paper itself

Abstract

Perivascular-resident macrophage-like melanocytes (PVM/Ms) can upregulate the expression of tight junction-related proteins in endothelial cells (ECs) by secreting pigment epithelial-derived factor (PEDF), and thereby regulate the permeability of the intrastrial fluid-blood barrier critical for maintaining inner ear homeostasis. This study aimed to investigate the effects of long non-coding RNA (lncRNA) Rian on cell growth of PVM/Ms and PVM/Ms regulation of intrastrial fluid-blood barrier integrity mediated by PEDF. Rian was downregulated in the aged cochlea from 12-month-old C57BL/6 mice. Rian overexpression inhibited cell apoptosis and promoted cell viability of hypoxia-injured PVM/Ms as well as increased the concentration and expression of PEDF secreted by PVM/Ms. In contrast, Rian silencing exerted the opposite effects. Furthermore, in a cell co-culture model of ECs and PVM/Ms, Rian overexpression in PVM/Ms increased the expression of the junction-associated proteins in co-cultured ECs, and this effect was abrogated by blockade of PEDF by anti-PEDF in PVM/Ms. Further mechanistical investigation revealed that Rian promoted STAT3 nuclear translocation and activation by binding to FUS, and thereby promoted the secretion of PEDF. Collectively, Rian attenuates PVM/Ms injury and strengthens the ability of PVM/Ms to maintain the integrity of the endothelial barrier by promoting PEDF expression.

Indexed as

AnimalsApoptosisCells, CulturedCell SurvivalCochleaEndothelial CellsEye ProteinsGene ExpressionMelanocytesMiceMice, Inbred C57BLNerve Growth FactorsNuclear ProteinsPigment Epithelium-Derived FactorProtein BindingRNA-Binding Protein FUSEye ProteinsFUS protein, mouseNerve Growth FactorsNuclear ProteinsPigment Epithelium-Derived FactorRian protein, mouseRNA-Binding Protein FUSRNA, Long NoncodingSerpinsStat3 protein, mouseSTAT3 Transcription FactorTight Junction ProteinsAge-related hearing lossPerivascular-resident macrophage-like melanocytesPigment epithelial-derived factorRian

Identifiers

PMID33768511
OpenAlexW3137841390

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.