ArticleScientific reports2021
Deep viral blood metagenomics reveals extensive anellovirus diversity in healthy humans.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
- Viral metagenomic analysis of the blood virome in patients with multiple autoimmune diseases.Virology journal · 2026Article
- Diverse anelloviruses identified in wild geladas (Theropithecus gelada).Archives of virology · 2026Article
- Anellovirus protein encoded byProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Infant gut anellovirus composition associates with early life factors and childhood atopic disease.Nature communications · 2025Article
- Comparative viral load analysis of torque Teno virus and Torque teno mini virus in COVID-19 infected and non-infected individuals in the United Arab Emirates.Molecular biology reports · 2025Article
- Viral Metagenomic Next-Generation Sequencing for One Health Discovery and Surveillance of (Re)Emerging Viruses: A Deep Review.International journal of molecular sciences · 2025Review
- Anellovirus species in the serum of acute febrile patients from Mato Grosso, central Western Brazil, 2019.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Anellovirus abundance as an indicator for viral metagenomic classifier utility in plasma samples.Virology journal · 2025Article
- The viral landscape in metastatic solid cancers.Heliyon · 2025Article
- The gut and circulating virome: emerging players in aging and longevity.Frontiers in aging · 2025Review
- Expanding the genomic diversity of human anelloviruses.Virus evolution · 2025Article
- Development and performance evaluation of a clinical metagenomics approach for identifying pathogens in the whole blood from patients with undifferentiated fever.Frontiers in cellular and infection microbiology · 2025Article
- Blood DNA virome associates with autoimmune diseases and COVID-19.Nature genetics · 2025Article
- A retrospective study revealing complex viral diversity and a substantial burden of HPV infection in SARS-CoV-2 positive individuals, Sierra Leone.Virology journal · 2024Article
- Long-Read Nanopore-Based Sequencing of Anelloviruses.Viruses · 2024Article
- Full annotation of viral metagenomics in different components from Chinese blood donors using next-generation sequencing.Blood transfusion = Trasfusione del sangue · 2024Article
- The mysterious anelloviruses: investigating its role in human diseases.BMC microbiology · 2024Review
- Pegivirus Detection in Cerebrospinal Fluid from Patients with Central Nervous System Infections of Unknown Etiology in Brazil by Viral Metagenomics.Microorganisms · 2023Article
- Expansion ofViruses · 2023Article
- Human Anelloviruses: Influence of Demographic Factors, Recombination, and Worldwide Diversity.Microbiology spectrum · 2023Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human blood metagenomics has revealed the presence of different types of viruses in apparently healthy subjects. By far, anelloviruses constitute the viral family that is more frequently found in human blood, although amplification biases and contaminations pose a major challenge in this field. To investigate this further, we subjected pooled plasma samples from 120 healthy donors in Spain to high-speed centrifugation, RNA and DNA extraction, random amplification, and massive parallel sequencing. Our results confirm the extensive presence of anelloviruses in such samples, which represented nearly 97% of the total viral sequence reads obtained. We assembled 114 different viral genomes belonging to this family, revealing remarkable diversity. Phylogenetic analysis of ORF1 suggested 28 potentially novel anellovirus species, 24 of which were validated by Sanger sequencing to discard artifacts. These findings underscore the importance of implementing more efficient purification procedures that enrich the viral fraction as an essential step in virome studies and question the suggested pathological role of anelloviruses.
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