ArticleScientific reports2021
eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 71 citations in OpenAlex.
- Trends in Aquatic Environmental DNA Research in Alaska.Ecology and evolution · 2026Review
- Properties and Limitations of eDNA Substrates for Terrestrial Animal Monitoring.Molecular ecology resources · 2026Review
- DNA metabarcoding on roadkill stomach contents reveals the breadth of species present in bobcat diets.PloS one · 2026Article
- Metabarcoding Close to Home: Songbird Nests as eDNA Aggregators for Trophic Ecology and Biodiversity Studies.Ecology and evolution · 2025Article
- eDNA Adoption: Weighing the Benefits and Challenges from Quebec Potential End-Users' Perspective.Environmental management · 2025Article
- Review
- First national survey of terrestrial biodiversity using airborne eDNA.Scientific reports · 2025Article
- Using grass inflorescence as source material for biomonitoring through environmental DNA metabarcoding.Molecular biology reports · 2024Article
- Molecular analysis of scats revealed diet and prey choice of grey wolves and Eurasian lynx in the contact zone between the Dinaric Mountains and the Alps.Frontiers in zoology · 2024Article
- Arthropod diversity in the alpine tundra using metabarcoding: Spatial and temporal differences in alpha- and beta-diversity.Ecology and evolution · 2024Article
- Article
- eDNA Metabarcoding- and Microscopic Analysis for Diet Determination in Waterfowl, a Comparative Study in Vejlerne, Denmark.Biology · 2023Article
- Molecular Diet Analysis of Asian Clams for Supplementary Biodiversity Monitoring: A Case Study of Nakdong River Estuary.Biology · 2023Article
- Herbivorous dietary selection shown by hawfinch (Royal Society open science · 2023Article
- Diet of a threatened rattlesnake (eastern massasauga) revealed by DNA metabarcoding.Ecology and evolution · 2023Article
- DNA metabarcoding reveals the dietary profiles of a benthic marine crustacean, Nephrops norvegicus.PloS one · 2023Article
- Focal vs. fecal: Seasonal variation in the diet of wild vervet monkeys from observational and DNA metabarcoding data.Ecology and evolution · 2022Article
- Interspecific coprophagia by wild red foxes: DNA metabarcoding reveals a potentially widespread form of commensalism among animals.Ecology and evolution · 2022Article
- Pollinator nutrition and its role in merging the dual objectives of pollinator health and optimal crop production.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- DNA metabarcoding reveals a broad dietary range for Tasmanian devils introduced to a naive ecosystem.Ecology and evolution · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors at 6 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With an accelerating negative impact of anthropogenic actions on natural ecosystems, non-invasive biodiversity assessments are becoming increasingly crucial. As a consequence, the interest in the application of environmental DNA (eDNA) survey techniques has increased. The use of eDNA extracted from faeces from generalist predators, have recently been described as "biodiversity capsules" and suggested as a complementary tool for improving current biodiversity assessments. In this study, using faecal samples from two generalist omnivore species, the Eurasian badger and the red fox, we evaluated the applicability of eDNA metabarcoding in determining dietary composition, compared to macroscopic diet identification techniques. Subsequently, we used the dietary information obtained to assess its contribution to biodiversity assessments. Compared to classic macroscopic techniques, we found that eDNA metabarcoding detected more taxa, at higher taxonomic resolution, and proved to be an important technique to verify the species identification of the predator from field collected faeces. Furthermore, we showed how dietary analyses complemented field observations in describing biodiversity by identifying consumed flora and fauna that went unnoticed during field observations. While diet analysis approaches could not substitute field observations entirely, we suggest that their integration with other methods might overcome intrinsic limitations of single techniques in future biodiversity surveys.
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