Evidence map›Paper›PMID 33765273›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Quantitative Comparisons of Translation Activity by Ribosome Profiling with Internal Standards.

Yinuo J Wang, Wendy V Gilbert

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Advances in ribosome profiling technologies.Biochemical Society transactions · 2025
    Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Yinuo J WangDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA.
Wendy V GilbertDepartment of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, USA. wendy.gilbert@yale.edu.

Funding

Ribosome Specialization and RegulationR01GM094303 · NIGMS · YALE UNIVERSITY · PI GILBERT, WENDY VICTORIA · 2012 to 2016
$1.5M
Translational Control by 5'-untranslated regionsR01GM132358 · NIGMS · YALE UNIVERSITY · PI GILBERT, WENDY VICTORIA · 2019 to 2022
$1.4M
NIGMS NIH HHS R01 GM094303NIGMS NIH HHS R01 GM132358
6 · The paper itself

Abstract

Ribosome profiling is a genome-wide approach to map the positions of ribosomes on messenger RNAs. The abundance of ribosome-protected fragments can be used within condition to compare relative translation activities between different transcripts and between distinct conditions for the same transcript. A unified and routine method is currently lacking, however, to normalize between conditions for differences in global translation levels. Here we describe experimental and computational methods to use an orthogonal species spike-in, or internal standard, to enable absolute comparisons of translation activity between conditions. This simple modification of standard ribosome profiling provides a robust approach for accurately interpreting the effects of diverse genetic, chemical, and environmental perturbations of translation.

Indexed as

Computational BiologyGene Expression ProfilingHeLa CellsHigh-Throughput Nucleotide SequencingHumansProtein BiosynthesisRibosomesRNA, MessengerSequence Analysis, RNARNA, MessengerNext-generation sequencingRibosome profilingSpike-in normalizationTranslation

Identifiers

PMID33765273

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.