Evidence map›Paper›PMID 33763113›Full record

ArticleFrontiers in genetics2021

Genetic Identification of Edible Bird's Nest in Thailand Based on ARMS-PCR.

Dongyong Lv, Yaohua Fan, Wanhua Zhong, Piyanuch Lonan, Kunfeng Liu, Maoyong Wu, Yina Wu, Yueliang Liang, Xiaoping Lai, Geng Li and 1 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Development of a tetra-primer ARMS-PCR for identification of sika and red deer and their hybrids.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Dongyong LvSchool of Nursing, Guangzhou University of Chinese Medicine, Guangzhou, China.
Yaohua FanShenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Guangzhou University of Chinese Medicine, Shenzhen, China.
Wanhua ZhongSchool of Physical Education and Health, Guangzhou University of Chinese Medicine, Guangzhou, China.
Piyanuch LonanLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Kunfeng LiuLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Maoyong WuSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Yina WuSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Yueliang LiangGuangzhou Tongkang Pharmaceutical Co., Ltd., Guangzhou, China.
Xiaoping LaiGuangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.
Geng LiLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Liangwen YuGuangdong Yunfu Vocational College of Chinese Medicine, Yunfu, China.
Guangzhou University of Chinese Medicine · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Edible bird's nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic information of EBN, a key part of molecular biology, has yet to be reported in Thailand. It is necessary to explore the genetic information of EBN in Thailand based on a quick and simple method to help protect the rights and interests of consumers. This research aimed to systematically evaluate different methods of extracting EBN DNA to improve the efficiency of the analysis of cytochrome b (Cytb) and NADH dehydrogenase subunit 2 (ND2) gene sequences, the establishment of phylogenetic trees, and the genetic information of EBN in Thailand. Additionally, we aimed to develop a quick and simple method for identifying EBN from different species based on the genetic information and amplification-refractory mutation system PCR (ARMS-PCR). By comparing the four methods [cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), kit and guanidinium isothiocyanate methods] for EBN extraction, we found that the guanidinium isothiocyanate method was the optimal extraction method. Phylogenetic trees generated on the basis of Cytb and ND2 gene analyses showed that 26 samples of house EBN and 4 samples of cave EBN came from

Indexed as

Aerodramus fuciphagusAerodramus maximusARMS-PCRedible bird’s nestgenetic informationguanidinium isothiocyanateND2

Identifiers

PMID33763113
PMCPMC7983251
OpenAlexW3135329845

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.