ArticleScientific reports2021
Evaluation of 16S rRNA primer sets for characterisation of microbiota in paediatric patients with autism spectrum disorder.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- NAP: an open source pipeline for cross-domain microbiome profiling using Nanopore sequencing-derived amplicon data.BMC bioinformatics · 2026Article
- Isolation and screening of silicate-solubilizing bacteria for enhanced desilicification of silica-containing agro-residues.Biodegradation · 2026Article
- The microbiota-gut-brain axis as a modulator of symptom expression in autism spectrum disorder, with exploratory insights into ADHD: evidence from a structured narrative review on paediatric population.Frontiers in child and adolescent psychiatry · 2026Review
- Garvicin Q-producing Lactococcus garvieae LG3092: its role in mitigating dysbiosis and H. pylori-associated gastritis.NPJ biofilms and microbiomes · 2025Article
- Biodiversity and Biological Interactions of Actinobacteria Associated with Deep Sea and Intertidal Marine Invertebrates.Marine drugs · 2025Article
- Protective Effects of Propolis Supplementation on Aflatoxin B1-Induced Oxidative Stress, Antioxidant Status, Intestinal Barrier Damage, and Gut Microbiota in Rats.Molecular nutrition & food research · 2025Article
- The molecular basis of Staphylococcus aureus resistance to linezolid and the role of silver nanoparticles in attenuating linezolid resistance.Molecular biology reports · 2025Article
- Metagenomic analysis reveals methanogenic and other archaeal genes in the digestive tract of invasive Japanese beetle larvae and associated soil.Frontiers in microbiology · 2025Article
- Can Gut Microbiota Analysis RevealMicroorganisms · 2024Article
- Analysis of Characteristics of Bovine-Derived Non-EnterotoxigenicMicroorganisms · 2024Article
- Uncovering microbiomes of the rice phyllosphere using long-read metagenomic sequencing.Communications biology · 2024Article
- Correlation between symptoms and cognitive function changes in patients with primary insomnia and pathways in gut microbiota.Biochemistry and biophysics reports · 2024Article
- Optimized bacterial community characterization through full-length 16S rRNA gene sequencing utilizing MinION nanopore technology.BMC microbiology · 2024Article
- Daily occupational exposure in swine farm alters human skin microbiota and antibiotic resistome.iMeta · 2024Article
- Hyperthermophile diversity microbes in the Calientes geothermal field, Tacna, Peru.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Article
- A Two-Step Single Plex PCR Method for Evaluating Key Colonic Microbiota Markers in Young Mexicans with Autism Spectrum Disorders: Protocol and Pilot Epidemiological Application.Diagnostics (Basel, Switzerland) · 2023Article
- The key players of dysbiosis in Noma disease; A systematic review of etiological studies.Frontiers in oral health · 2023Review
- Gut microbiota from essential tremor patients aggravates tremors in mice.Frontiers in microbiology · 2023Article
- Prevalence of antibiotic-resistant Gram-negative bacteria having extended-spectrum β-lactamase phenotypes in polluted irrigation-purpose wastewaters from Indian agro-ecosystems.Frontiers in microbiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
intestinal microbiota is becoming a significant marker that reflects differences between health and disease status also in terms of gut-brain axis communication. Studies show that children with autism spectrum disorder (ASD) often have a mix of gut microbes that is distinct from the neurotypical children. Various assays are being used for microbiota investigation and were considered to be universal. However, newer studies showed that protocol for preparing DNA sequencing libraries is a key factor influencing results of microbiota investigation. The choice of DNA amplification primers seems to be the crucial for the outcome of analysis. In our study, we have tested 3 primer sets to investigate differences in outcome of sequencing analysis of microbiota in children with ASD. We found out that primers detected different portion of bacteria in samples especially at phylum level; significantly higher abundance of Bacteroides and lower Firmicutes were detected using 515f/806r compared to 27f/1492r and 27f*/1495f primers. So, the question is whether a gold standard of Firmicutes/Bacteroidetes ratio is a valuable and reliable universal marker, since two primer sets towards 16S rRNA can provide opposite information. Moreover, significantly higher relative abundance of Proteobacteria was detected using 27f/1492r. The beta diversity of sample groups differed remarkably and so the number of observed bacterial genera.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.