ArticlePloS one2021
A faster and less costly alternative for RNA extraction of SARS-CoV-2 using proteinase k treatment followed by thermal shock.
Article in PloS one, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Separation of Magnetic Microparticles With Different Molecular Surface Functionalizations by Close-to-Surface Traveling-Wave Magnetophoresis.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Innovations in reagent manufacturing to support the global diagnostics supply chain.Frontiers in public health · 2026Article
- Degradation of Preformed Gram-Positive and Gram-Negative Bacterial Biofilms Using Disintegrated and Intact Phages.PHAGE (New Rochelle, N.Y.) · 2025Article
- Development of a Novel Loop-Mediated Isothermal Amplification (LAMP) for Rapid Diagnosis of Newcastle Disease in Field and Resource-Limited Areas.Veterinary medicine (Auckland, N.Z.) · 2025Article
- Successful application of modified crude DNA extraction from muscle tissues for various types of PCR amplifications.Molecular biology reports · 2024Article
- An Open One-Step RT-qPCR for SARS-CoV-2 detection.PloS one · 2024Article
- Promise and perils of paper-based point-of-care nucleic acid detection for endemic and pandemic pathogens.Lab on a chip · 2023Review
- SARS-CoV-2 genotyping and sequencing following a simple and economical RNA extraction and storage protocol.PloS one · 2023Article
- Challenges of SARS-CoV-2 genomic surveillance in India during low positivity rate scenario.Frontiers in public health · 2023Article
- Dry Swab-Based Nucleic Acid Extraction vs. Spin Column-Based Nucleic Acid Extraction for COVID-19 RT-PCR Testing: A Comparative Study.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2023Article
- Simple and low-cost nucleic acid extraction methods for detection of SARS-CoV2 in self-collected saliva and dry oral swabs.IJID regions · 2022Article
- Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads.Diagnostics (Basel, Switzerland) · 2022Article
- Strategies That Facilitate Extraction-Free SARS-CoV-2 Nucleic Acid Amplification Tests.Viruses · 2022Review
- Advancements in Testing Strategies for COVID-19.Biosensors · 2022Review
- Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification.Viruses · 2022Article
- Emergence of SARS-CoV-2 Delta Variant, Benin, May-July 2021.Emerging infectious diseases · 2022Article
- SwabExpress: An End-to-End Protocol for Extraction-Free COVID-19 Testing.Clinical chemistry · 2021Article
- SwabExpress: An end-to-end protocol for extraction-free COVID-19 testing.bioRxiv : the preprint server for biology · 2021Article
- Different Respiratory Samples for COVID-19 Detection by Standard and Direct Quantitative RT-PCR: A Literature Review.Iranian journal of pharmaceutical research : IJPR · 2021Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
One of the biggest challenges during the pandemic has been obtaining and maintaining critical material to conduct the increasing demand for molecular tests. Sometimes, the lack of suppliers and the global shortage of these reagents, a consequence of the high demand, make it difficult to detect and diagnose patients with suspected SARS-CoV-2 infection, negatively impacting the control of virus spread. Many alternatives have enabled the continuous processing of samples and have presented a decrease in time and cost. These measures thus allow broad testing of the population and should be ideal for controlling the disease. In this sense, we compared the SARS-CoV-2 molecular detection effectiveness by Real time RT-PCR using two different protocols for RNA extraction. The experiments were conducted in the National Institute of Health (INS) from Peru. We compared Ct values average (experimental triplicate) results from two different targets, a viral and internal control. All samples were extracted in parallel using a commercial kit and our alternative protocol-samples submitted to proteinase K treatment (3 μg/μL, 56°C for 10 minutes) followed by thermal shock (98°C for 5 minutes followed by 4°C for 2 minutes); the agreement between results was 100% in the samples tested. In addition, we compared the COVID-19 positivity between six epidemiological weeks: the initial two in that the Real time RT-PCR reactions were conducted using RNA extracted by commercial kit, followed by two other using RNA obtained by our kit-free method, and the last two using kit once again; they did not differ significantly. We concluded that our in-house method is an easy, fast, and cost-effective alternative method for extracting RNA and conducing molecular diagnosis of COVID-19.
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Registered trials
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